LOCUS       sequence-150676-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A single vector AAV-Cas9 system containing SaCas9 under EFs 
            promoter, gRNAs targeting Dmd introns 22 and 23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cggttctaga gcgctgccac catgaaaagg aactacattc tggggctgga catcgggatt
       61 acaagcgtgg ggtatgggat tattgactat gaaacaaggg acgtgatcga cgcaggcgtc
      121 agactgttca aggaggccaa cgtggaaaac aatgagggac ggagaagcaa gaggggagcc
      181 aggcgcctga aacgacggag aaggcacaga atccagaggg tgaagaaact gctgttcgat
      241 tacaacctgc tgaccgacca ttctgagctg agtggaatta atccttatga agccagggtg
      301 aaaggcctga gtcagaagct gtcagaggaa gagttttccg cagctctgct gcacctggct
      361 aagcgccgag gagtgcataa cgtcaatgag gtggaagagg acaccggcaa cgagctgtct
      421 acaaaggaac agatctcacg caatagcaaa gctctggaag agaagtatgt cgcagagctg
      481 cagctggaac ggctgaagaa agatggcgag gtgagagggt caattaatag gttcaagaca
      541 agcgactacg tcaaagaagc caagcagctg ctgaaagtgc agaaggctta ccaccagctg
      601 gatcagagct tcatcgatac ttatatcgac ctgctggaga ctcggagaac ctactatgag
      661 ggaccaggag aagggagccc cttcggatgg aaagacatca aggaatggta cgagatgctg
      721 atgggacatt gcacctattt tccagaagag ctgagaagcg tcaagtatgc ttataacgca
      781 gatctgtaca acgccctgaa tgacctgaac aacctggtca tcaccaggga tgaaa
//
