LOCUS       sequence-150678-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNAs (for SpCas9) targeting 5' of Ai9 stop cassette and 
            Dmd intron 22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pCRII-Topo U6-SpAi9L-U6SpDMDL
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        134..382
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     305..324
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(501..525)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt gttcgtctcg
       61 tccctgtaca aaaaagcagg ctttaaagga accaattcag tcgactggat ccggtaccaa
      121 ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
      181 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
      241 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      301 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      361 cttgtggaaa ggacgaaaca ccgaaagaat tgatttgata ccggtttaag agctatgctg
      421 gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      481 agtcggtgct ttttttctag acccagcttt cttgtacaaa gttggcatta tgagggggag
      541 acgagaaggg cgaattctgc agcgtccctg tacaaaaaag caggctttaa aggaaccaat
      601 tcagtcgact ggatccggta ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc
      661 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt
      721 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
      781 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg c
//
