LOCUS       sequence-150679-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium 
            acetobutylicum ATCC 824.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren 
            X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S
  TITLE     CRISPR-based genome editing and expression control systems in 
            Clostridium acetobutylicum and Clostridium beijerinckii.
  JOURNAL   Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
  PUBMED    27213844
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2016 May 23. doi: 10.1002/biot.201600053."
COMMENT     Sequence Label: pdCASclos
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(24..58)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
ORIGIN
        1 aactattatc atctgcaatt aaaactagta ttatacctag gactgagcta gctgtcaact
       61 gcagccaagc ttaatcgcat ttcatagatt gacctcccaa taactacgtg gtgttattgg
      121 gaggtcaatc tatttcattt gcctcttgct caaagttccc aaattcgagt aagaggtatt
      181 tttgtttttg gtcgtcgcct ctcattagta gttcagggtt taacattaat actccagttt
      241 ttctttttat aatatttcct tcttctaaga ttttaagtgt tgttattact gtttgtagac
      301 ttgttcctgt agcttttgct atttctcttg ttgtagctat cattgtattg ttacttaagt
      361 ggacattatc taggatatag ttaacgattt taagtttttt tccgccaatc atatctaaca
      421 tacttattaa ttgcactata tatgccttta cgaagttacc agacgtttgt ttacggtata
      481 acttgtctac ctctatgact tctccacttt cttcgtctat gagcctctga gagcctttat
      541 agactgttcc atatctttct ttcatctttt tctcactcct tattttaaac tattctaact
      601 atatcataac tgttctaaaa aaaaaagaac atttgttaaa agaaattaga acaaaatgag
      661 tgaaaaatta gaacaaacaa attccttata aaccttatca tctcaaccta tattaagatt
      721 ttacctagtt gaatcttctt ttctatataa agcgtcggag catatcaggg ggttatctaa
      781 cgtaaatgct acccttcggc tcgctttcgc tcggcattga cgtcagatac tgcac
//
