LOCUS       sequence-150682-         501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNAs (for SpCas9) targeting 3' of Ai9 stop cassette and 
            Dmd intron 23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pCRII-Topo U6-SpAi9R-U6-SpDMDR
FEATURES             Location/Qualifiers
     source          1..501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..55
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(176..424)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(234..253)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(404..424)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 attcgccctt ctcgtctccc cctcataatg ccaactttgt acaagaaagc tgggtctaga
       61 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttatttaaac
      121 ttgctatgct gtttccagca tagctcttaa accggcttac ctgaaatttt cgaacggtgt
      181 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
      241 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
      301 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa
      361 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
      421 cctcttcctg cccgaccttg gtaccggatc cagtcgactg aattggttcc tttaaagcct
      481 gcttttttgt acagggacgc t
//
