LOCUS       sequence-150683-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNAs (for SpCas9) targeting 3' of Ai9 stop cassette and 
            Dmd intron 23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pCRII-Topo U6-SpAi9R-U6-SpDMDR
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        128..376
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     299..318
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(495..519)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcggaccct agtaacggcc gccagtgtgc tggaattcgc ccttgttcgt ctcgtccctg
       61 tacaaaaaag caggctttaa aggaaccaat tcagtcgact ggatccggta ccaaggtcgg
      121 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
      181 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
      241 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      301 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      361 gaaaggacga aacaccggta tgctatacga agttattgtt taagagctat gctggaaaca
      421 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      481 tgcttttttt ctagacccag ctttcttgta caaagttggc attatgaggg ggagacgaga
      541 agggcgaatt ctgcagcgtc cctgtacaaa aaagcaggct ttaaaggaac caattcagtc
      601 gactggatcc ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata
      661 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
      721 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      781 ttt
//
