LOCUS       sequence-150684-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of cas9 and inducible expression of lambda 
            RED and sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S
  TITLE     A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome 
            editing in Escherichia coli.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2021 Apr 15;53(5):620-627. doi:
            10.1093/abbs/gmab036.
  PUBMED    33764372
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Acta Biochim Biophys Sin (Shanghai)"; date: "2021-04-15- 15"; 
            volume: "53"; issue: "5"; pages: "620-627"
COMMENT     Sequence Label: pEcCas
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(25..143)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
ORIGIN
        1 aacggttgga ctcaagacga tagttacgac cagtctaaaa agcgcctgaa ttcgcgacct
       61 tctcgttact gacaggaaaa tgggccattg gcaaccaggg aaagatgaac gtgatgatgt
      121 tcacaatttg ctgaattgtg gtgatgtgat gctcaccgca tttcctgaaa attcacgctg
      181 tatcttgaaa aatcgacgtt ttttacgtgg ttttccgtcg aaaatttaag gtaagaacct
      241 gacctcgtga ttactatttc gccgtgttga cgacatcagg aggccagtat gaccgtatta
      301 catagtgtgg atttttttcc gtctggtaac gcgtccgtgg cgatagaacc ccggctcccg
      361 caggcggatt ttcctgaaca tcatcatgat tttcatgaaa ttgtgattgt cgaacatggc
      421 acgggtattc atgtgtttaa tgggcagccc tataccatca ccggtggcac ggtctgtttc
      481 gtacgcgatc atgatcggca tctgtatgaa cataccgata atctgtgtct gaccaatgtg
      541 ctgtatcgct cgccggatcg atttcagttt ctcgccgggc tgaatcagtt gctgccacaa
      601 gagctggatg ggcagtatcc gtctcactgg cgcgttaacc acagcgtatt gcagcaggtg
      661 cgacagctgg ttgcacagat ggaacagcag gaaggggaaa atgatttacc ctcgaccgcc
      721 agtcgcgaga tcttgtttat gcaattactg ctcttgctgc gtaaaagcag tttgcaggag
      781 aacctggaaa acagcgcatc acgtctcaac ttgcttctgg cctggctgga ggaccat
//
