LOCUS       sequence-150685-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of cas9 and inducible expression of lambda 
            RED and sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S
  TITLE     A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome 
            editing in Escherichia coli.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2021 Apr 15;53(5):620-627. doi:
            10.1093/abbs/gmab036.
  PUBMED    33764372
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Acta Biochim Biophys Sin (Shanghai)"; date: "2021-04-15- 15"; 
            volume: "53"; issue: "5"; pages: "620-627"
COMMENT     Sequence Label: pEcCas
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatgt ctagattaag
       61 aaataatctt catctaaaat atacttcagt cacctcctag ctgactcaaa tcaatgcgtg
      121 tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact tcttttgtag
      181 acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg ggagctccaa
      241 gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt ggtttgtctc
      301 tatgtttgtt atatgcacta agaactttat ctaaattggc atctgctaaa ataacacgct
      361 tagaaaattc actgatttgc tcaataatct catctaaata atgcttatgc tgctccacaa
      421 acaattgttt ttgttcgtta tcttctggac tacccttcaa cttttcataa tgactagcta
      481 aatataaaaa attcacatat ttgcttggca gagccagctc atttcctttt tgtaattctc
      541 cggcactagc cagcatccgt ttacgaccgt tttctaactc aaaaagacta tatttaggta
      601 gtttaatgat taagtctttt ttaacttcct tatatccttt agcttctaaa aagtcaatcg
      661 gatttttttc aaaggaactt ctttccataa ttgtgatccc tagtaactct ttaacggatt
      721 ttaacttctt cgatttccct ttttccacct tagcaaccac taggactgaa taagctaccg
//
