LOCUS       sequence-150687-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing SaCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner
            C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church 
            GM, Wagers AJ
  TITLE     In vivo gene editing in dystrophic mouse muscle and muscle stem 
            cells.
  JOURNAL   Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177.
            Epub 2015 Dec 31.
  PUBMED    26721686
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad5177"; journalName: "Science"; date: 
            "2016-01-22- 22"; volume: "351"; issue: "6271"; pages: "407-11"
COMMENT     Sequence Label: pZac2.1 SV40-CMV-SaCas9-3xNLS
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..27)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(34..54)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(58..78)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(82..102)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcgtaatct ggaacatcgt atgggtagga tccgactttt ctttttttct tgggatctac
       61 ctttctcttc ttttttggat caaccttcct cttcttcttg gggtcgccct ttttgataat
      121 ctgagggtgc tttttgctct tcacctcata caggtttccc agaatgtcgg ttgagtactt
      181 tttgatactc tgagtcttag aggcaattgt tttgataatt cgaggggggc gcttatcatt
      241 catgttttcc agatactctc ggtaagtgat gtcaatcata ttcacttcaa tgcggttcag
      301 cagatcattg ttcaccccga tgaccctata cagttcgcca ttgatcttaa tcaggtcgtt
      361 gttgtaaaag gaggcgatga actctgcctg gttgctaatc tttttcagct ttttagcctc
      421 ttcgtagcac ttgctattca cttcatagta gttctccttt ttgatgacat ccagattctt
      481 gacagtcaca aatttataca cgccgttgtc cagatagaca tcgaatctgt atggcttcag
      541 tgacagcttg accaccttgt tgcgactgtt agggtaatcg tctgtgatgt ccagatgggc
      601 attcagcttg ttcccatagt acttgatctt cttgatcacg gggccattat cctttttgct
      661 atacttggtc aggtagttcc cagtctcttc atagtactta tacagtgggt tcttctcgtc
      721 gccgtactgc tccataatca gcttcagttt ctgatatgtc tgaggatcat ggtggtacat
      781 cagcagcttc tcgggacttt tgttgatcag ctttttcagc ttgtcattat ctttgtcgta
      841 cagtccgttc agattgttca caatcaggg
//
