LOCUS       sequence-150688-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression and retroviral Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Srikanth S, Jung HJ, Ribalet B, Gwack Y
  TITLE     The intracellular loop of Orai1 plays a central role in fast 
            inactivation of Ca2+ release-activated Ca2+ channels.
  JOURNAL   J Biol Chem. 2010 Feb 12;285(7):5066-75. doi: 
            10.1074/jbc.M109.072736. Epub 2009 Dec 10.
  PUBMED    20007711
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.072736"; journalName: "J Biol Chem"; date: 
            "2010-02-12- 12"; volume: "285"; issue: "7"; pages: "5066-75"
COMMENT     Sequence Label: MO70 - Orai1 EC-Flag
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(85..108)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(445..468)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      797..806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acagggccag gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac
       61 cggaattact cgcgacaatt ctcacttgtc atcgtcgtcc ttgtagtcag cggccgcaat
      121 atcgatctgg ttaacgtaga attcctagtg gctgccgggc gtcagggggt ggtcccctct
      181 gtggtccagc tggtcctgta agcgggcaaa ctccgccagc tcgttgagct cctggaactg
      241 tcggtcggtc ttatggctaa ccagtgagcg gtagaagtgg acggcgaaga cgataaagat
      301 caggccgaag ggcaccatga tggtggtcga ggcgatggca gctgcctggc ccggggtgat
      361 gccgctggtg ctgacgttgg ctgctgcgcc actggcgggg ggcttgctgg tgggccttgg
      421 ctggccacgc gtgctgccgc tgcccttgtc atcgtcgtcc ttgtagtcgc tgccgctgcc
      481 gtcgactggc tgcttcttga ggggcaagaa cttgacccag cagagcagca ccacctcagc
      541 taggaagagc agcgtgccaa tgacggtgga gaaggcccag gccagctcga tgtggcggtg
      601 catgcgctca tggggggact ccttgaccga gttgagattg tgcacgttgc tcaccgcctc
      661 gatgttgggc aggatgcagg tgctgatcat gagcgcaaac aggtgcacag ccaccagcac
      721 tgtggtgcag gcactgaagg cgatgagcag ccccggtggg tagtcgtggt cagcgtccag
      781 ctgcacctcc accattgcca ccatggcgaa gccggagagc agagccgagg tccggctgga
      841 ggctttaagc ttggcgcggc tcaagtagag cttgcgccag gacagcgcct gcatggagtg
      901 ctcgttgagg ctcatcacct c
//
