LOCUS       sequence-150693-        3324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded melanosome-localized pH sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ambrosio AL, Boyle JA, Aradi AE, Christian KA, Di Pietro SM
  TITLE     TPC2 controls pigmentation by regulating melanosome pH and size.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 17;113(20):5622-7. doi: 
            10.1073/pnas.1600108113. Epub 2016 May 2.
  PUBMED    27140606
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1600108113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-17- 17"; volume: "113"; issue: "20"; pages: "5622-7"
COMMENT     Sequence Label: MELOPS
FEATURES             Location/Qualifiers
     source          1..3324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      769..778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             775..1482
                     /codon_start=1
                     /product="pH-sensitive monomeric red fluorescent protein 
                     derived from DsRed and mTangerine (Johnson et al., 2009)"
                     /label=mNectarine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFCYGSKAYVKHPADIPDYLKLSFPEGLNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTVGWEASTERMHPEDGALK
                     GEIMQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(920..938)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atgcatcttg agggcagaga cggcaggcgg taccccggcg cgccggcggt ggagctcctg
       61 cagacgtccg tgcccagcgg actcgctgaa cttgtggccg gcaagcgcag gcttcctcgg
      121 ggagccggtg gagctgaccc ctcgcactcc tgccccaggg gggctgccgg gcagagctct
      181 tgggctcctg caggccagga gtttgcttca ttcctcacaa aagggaggtc tcactcttct
      241 ttgccccaga tgtccagctc caggtctaaa gattcctgct ttacagaaaa cactcctttg
      301 ctgaggaatt ccttacagga gaaagggtca cggtgcatac ctgtttacca tccagagttc
      361 atcactgctg aagagtcttg ggaagacagc tctgctgact gggagcgaag atacctgcta
      421 agcagggagg tgtctggtct gtctgcatct gcctcctccg agaagggaga ccttctggac
      481 agcccgcaca tccgactccg tctttccaag ctgaggcgct gtgtgcagtg gctgaaagtc
      541 atgggcctgt ttgcctttgt ggtgctgtgt tctattttgt tcagcctata tccggatcaa
      601 ggaaagctct ggcagctgtt ggccttatca ccgctggaga actactccgt gaaccttagc
      661 agccacgtgg actccacgct gctgcaggtg gacctggcag gggccctagt ggccagtggg
      721 ccgagtcgtg gttctcctgg ttctggttct ggttctggtt ctccggtcgc caccatggtg
      781 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      841 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gtgagggcga gggccgcccc
      901 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      961 tgggacatcc tgtcccctca gttctgctac ggctccaagg cctatgtgaa gcaccccgcc
     1021 gacatccccg actacttgaa gctgtccttc cccgagggcc tcaattggga gcgcgtgatg
     1081 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     1141 ttcatctaca aggtgaagct gcgcggcact aacttcccct ccgacggccc cgtaatgcag
     1201 tgtcgtaccg tgggctggga ggcctccact gagcggatgc accccgagga cggcgccctg
     1261 aagggcgaga tcatgcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     1321 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcgacatc
     1381 aagttggaca tcctttccca caacgaggac tacaccatcg tggaactgta cgaacgcgct
     1441 gagggccgcc actccaccgg cggcatggac gagctgtaca agtccggccg gctcgagtct
     1501 ggttctggtt ctggttctgg ttctggtcct tctggtcctg ggagggaaga gcacatcgtg
     1561 gtggagctga cccaggctga cgctttgggc tccaggtggc ggcggccaca gcaggtcact
     1621 cacaactgga cggtgtattt aaatccgagg agaagcgagc actcagtgat gagcaggacc
     1681 tttgaggtac tgaccagaga gacggtgtcc atcagcatcc gggcctccct gcagcagacc
     1741 caggctgtcc ctcttttgat ggctcatcag tacctccgcg gaagtgtaga aacccaggtg
     1801 accatcgcga cggccatcct cgcgggcgtc tacgcgctga tcatatttga gatcgtgcac
     1861 agaactctgg cagccatgct gggttccctt gcagcactgg cagcactggc tgtgattggc
     1921 gatagaccca gcctgaccca tgtggtggag tggattgatt ttgagacgct ggccctgctg
     1981 tttggcatga tgatcttagt agccatattt tcagaaacgg gatttttcga ttattgtgct
     2041 gtaaaggcat accggctctc ccggggacgg gtgtgggcca tgatcatcat gctctgtctc
     2101 atcgcggccg tcctctctgc cttcttggac aacatcacca ccatgctcct cttcacgcct
     2161 gtgaccataa ggttgtgtga ggtgctcaac cttgatccaa gacaagtcct gattgcagaa
     2221 gtgatcttca caaacattgg aggagctgcc actgccatcg gggaccctcc aaatgtcatt
     2281 attgtttcca accaagagct gaggaagatg ggcctggact ttgccggatt cactgcacac
     2341 atgttcattg ggatttgtct tgttctcctg gtctgctttc cgctcctcag actcctttac
     2401 tggaacagaa agctttataa caaggaaccc agtgagattg ttgaactgaa gcacgagatt
     2461 cacgtctggc gcctgactgc tcagcgcatc agcccggcca gccgcgagga gacagctgtg
     2521 cgccgcctgc tgctggggaa ggtgctggca ctggagcacc tgctcgcccg gaggctgcac
     2581 accttccaca gacagatctc acaggaggac aaaaattggg agaccaatat ccaagaactc
     2641 caaaaaaagc ataggatatc tgacgggatt ctgctcgcca aatgcctgac agtgttggga
     2701 tttgttatct tcatgttttt cctcaattcg tttgtccctg gcattcatct tgatcttgga
     2761 tggattgcta ttctgggtgc catctggttg ctaattttag ctgatattca tgattttgag
     2821 ataattctac acagagtgga atgggcaacc cttctgtttt ttgcagcgct ctttgttctg
     2881 atggaggcat tggcacatct ccacttaata gaatatgttg gagaacaaac tgctttgcta
     2941 ataaagatgg tcccagagga gcagcgcctc atagccgcca ttgtcctggt ggtgtgggtc
     3001 tcagccctgg cgtcgtccct gattgacaac atcccgttca ctgctaccat gattcccgtg
     3061 ctcctgaacc tgagccacga ccctgaggtt ggcctgcccg caccgccgct catgtatgcc
     3121 ctggctttcg gtgcttgcct gggaggtaac gggacactga ttggcgcgtc agcaaacgtc
     3181 gtgtgtgcag ggattgcaga acagcatgga tatgggttct ccttcatgga atttttcagg
     3241 ctgggcttcc caatgatggt tgtgtcctgc actgttggga tgtgttatct ccttgtggct
     3301 catgtggtgg tgggatggaa ttaa
//
