LOCUS       sequence-150701-        7915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express of GCN5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Mao X, Gluck N, Li D, Maine GN, Li H, Zaidi IW, Repaka A, Mayo MW, 
            Burstein E
  TITLE     GCN5 is a required cofactor for a ubiquitin ligase that targets 
            NF-kappaB/RelA.
  JOURNAL   Genes Dev. 2009 Apr 1;23(7):849-61. doi: 10.1101/gad.1748409.
  PUBMED    19339690
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2009-04-1- 1"; volume: "23"; issue:
            "7"; pages: "849-61"
COMMENT     Sequence Label: pEBB Flag GCN5
FEATURES             Location/Qualifiers
     source          1..7915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        252..462
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        274..470
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      321..456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        557..1739
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          788..1730
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1687..1707
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1795..1818
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5049..5066)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5049..5065)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5058..5080)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5278..5733
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5365..5384)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5575..5596
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5750..5769)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5929..5947)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6015..6119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6120..6188,6189..6980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6338..6357)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7151..7739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7640..7659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7893..7910
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttc
      241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttgca
      481 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
      541 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      601 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
      661 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      721 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      781 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
      841 ttgcgtgcct tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc
      901 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
      961 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     1021 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     1081 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     1141 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     1201 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     1261 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     1321 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     1381 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     1441 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     1501 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     1561 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     1621 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     1681 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     1741 gaattctcta gagatccctc gacctcgaga tccattgtgc tggatctcac catggactac
     1801 aaagacgatg acgacaaggg atccactagt atggcggaac cttcccaggc cccgaccccg
     1861 gccccggctg cgcagccccg gccccttcag tccccagccc ctgccccaac tccgactcct
     1921 gcacccagcc cggcttcagc cccgattccg actcccaccc cggcaccagc ccctgcccca
     1981 gctgcagccc cagccggcag cacagggact ggggggcccg gggtaggaag tgggggggcc
     2041 gggagcgggg gggatccggc tcgacctggc ctgagccagc agcagcgcgc cagtcagagg
     2101 aaggcgcaag tccgggggct gccgcgcgcc aagaagcttg agaagctagg ggtcttctcg
     2161 gcttgcaagg ccaatgaaac ctgtaagtgt aatggctgga aaaaccccaa gccccccact
     2221 gcaccccgca tggatctgca gcaaccagct gcaaacctga gtgagctgtg ccgcagttgt
     2281 gagcacccct tggctgacca cgtatcccac ttggagaatg tgtcagagga tgagataaac
     2341 cgactgctgg ggatggtggt ggatgttgag aatctcttca tgtctgttca caaggaagag
     2401 gacacagaca ccaagcaggt ctatttctac ctcttcaagc tactgcggaa atgcatcctg
     2461 cagatgaccc ggcctgtggt ggaggggtcc ctgggcagcc ctccatttga gaaacctaat
     2521 attgagcagg gtgtgctgaa ctttgtgcag tacaagttta gtcacctggc tccccgggag
     2581 cggcagacga tgttcgagct ctcaaagatg ttcttgctct gccttaacta ctggaagctt
     2641 gagacacctg cccagtttcg gcagaggtct caggctgagg acgtggctac ctacaaggtc
     2701 aattacacca gatggctctg ttactgccac gtgccccaga gctgtgatag cctcccccgc
     2761 tacgaaacca ctcatgtctt tgggcgaagc cttctccggt ccattttcac cgttacccgc
     2821 cggcagctgc tggaaaagtt ccgagtggag aaggacaaat tggtgcccga gaagaggacc
     2881 ctcatcctca ctcacttccc caaattcctg tccatgctgg aggaggagat ctatggggca
     2941 aactctccaa tctgggagtc aggcttcacc atgccaccct cagaggggac acagctggtt
     3001 ccccggccag cttcagtcag tgcagcggtt gttcccagca cccccatctt cagccccagc
     3061 atgggtgggg gcagcaacag ctccctgagt ctggattctg caggggccga gcctatgcca
     3121 ggcgagaaga ggacgctccc agagaacctg accctggagg atgccaagcg gctccgtgtg
     3181 atgggtgaca tccccatgga gctggtcaat gaggtcatgc tgaccatcac tgaccctgct
     3241 gccatgctgg ggcctgagac gagcctgctt tcggccaatg cggcccggga tgagacagcc
     3301 cgcctggagg agcgccgcgg catcatcgag ttccatgtca tcggcaactc actgacgccc
     3361 aaggccaacc ggcgggtgtt gctgtggctc gtggggctgc agaatgtctt ttcccaccag
     3421 ctgccgcgca tgcctaagga gtatatcgcc cgcctcgtct ttgacccgaa gcacaagact
     3481 ctggccttga tcaaggatgg gcgggtcatc ggtggcatct gcttccgcat gtttcccacc
     3541 cagggcttca cggagattgt cttctgtgct gtcacctcga atgagcaggt caagggttat
     3601 gggacccacc tgatgaacca cctgaaggag tatcacatca agcacaacat tctctacttc
     3661 ctcacctacg ccgacgagta cgccatcggc tacttcaaaa agcagggttt ctccaaggac
     3721 atcaaggtgc ccaagagccg ctacctgggc tacatcaagg actacgaggg agcgacgttg
     3781 atggagtgtg agctgaatcc ccgcatcccc tacacggagc tgtcccacat catcaagaag
     3841 cagaaagaga tcatcaagaa gctgattgag cgcaaacagg cccagatccg caaggtctac
     3901 ccggggctca gctgcttcaa ggagggcgtg aggcagatcc ctgtggagag cgttcctggc
     3961 attcgagaga caggctggaa gccattgggg aaggagaagg ggaaggagct gaaggacccc
     4021 gaccagctct acacaaccct caaaaacctg ctggcccaaa tcaagtctca ccccagtgcc
     4081 tggcccttca tggagcctgt gaagaagtcg gaggcccctg actactacga ggtcatccgc
     4141 ttccccattg acctgaagac catgactgag cggctgcgaa gccgctacta cgtgacccgg
     4201 aagctctttg tggccgacct gcagcgggtc atcgccaact gtcgcgagta caaccccccg
     4261 gacagcgagt actgccgctg tgccagcgcc ctggagaagt tcttctactt caagctcaag
     4321 gagggaggcc tcattgacaa gtagggtacc atcgattgac tgactgagcg gccgcgactc
     4381 tagagtgagg gtccccacct gggacccttg agagtatcag gtctcccacg tgggagacaa
     4441 gaaatccctg tttaatattt aaacagcagt gttccccatc tgggtccttg cacccctcac
     4501 tctggcctca gccgactgca cagcggcccc tgcatcccct tggctgtgag gcccctggac
     4561 aagcagaggt ggccagagct gggaggcatg gccctggggt cccacgaatt tgctggggaa
     4621 tctcgttttt cttcttaaga cttttgggac atggtttgac tcccgaacat caccgacgcg
     4681 tctcctgttt ttctgggtgg cctcgggaca cctgccctgc ccccacgagg gtcaggactg
     4741 tgactctttt tagggccagg caggtgcctg gacatttgcc ttgctggacg gggactgggg
     4801 atgtgggagg gagcagacag gaggaatcat gtcaggcctg tgtgtgaaag gaagctccac
     4861 tgtcaccctc cacctcttca ccccccactc accagtgtcc cctccactgt cacattgtaa
     4921 ctgaacttca ggataataaa gtgtttgcct ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5041 aagaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5101 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5161 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     5221 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     5281 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5341 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5401 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5461 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     5521 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5581 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     5641 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5701 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     5761 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5821 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5881 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5941 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6001 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6061 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7261 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7681 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7801 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7861 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagc
//
