LOCUS       sequence-150721-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 548 / 634, Emission: 565 
            / 662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-mOrange-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(121..831)
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(201..224)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      828..837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
      121 ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
      181 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttgatgc cgacgatgta
      241 ggcgccgggc agctgcacgg acttcttggc cttgtaggtg gtcttgacct cggaggtgta
      301 gtggccgccg tccttcagct tcagcctcat cctgatctcg cccttcaggg cgccgtcctc
      361 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca tcacggggcc
      421 gtcggagggg aagttggtgc cgcgcatctt caccttgtag atgaactcgc cgtcctgcag
      481 tgaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg tagttcatca cgcgctccca
      541 cttgaagccc tcggggaagg acagcttgaa gtagtcgggg atgtcggcgg ggtgcttcac
      601 gtaggccttg gagccgtagg tgaagagagg ggacaggatg tcccaggcga agggcagggg
      661 gccgcccttg gtcaccttca gcttagcggt ctgaaagccc tcgtaggggc ggccctcgcc
      721 ctcgccctcg atctcgaact cgtggccgtt cacggtgccc tccatgcgca ccttgaagcg
      781 catgaactcc ttgatgatgg ccatattatt ctcctcgccc ttgctcacca tggtggcgac
      841 cggtggatcc cgggcccgcg ggtaccgtcg actgcagaat tcgaa
//
