LOCUS       sequence-150734-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  positive control for lncRNA expression assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  TITLE     pTREtight reporter plasmids--Roderic Guigo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTre-Tight-luciferase-Hottip (522)
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(524..541)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(775..794)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggcacc
       61 gagctcggat cccgccttta tcccagtggg gcccagaccc gcgcaaccag gcggggaggg
      121 gaggtgggcg cgcgattggg ttgcgatctg gagcagtggg gacaggtcag gaaccggcgc
      181 gtatttctgc agtgagacca caggacggac atcggcgcct tcggcttcga tggagttgcg
      241 attttgctct ttccagggaa acagtggcag ggtgtttgct gcttatcggt tcctgcggat
      301 atgcctgggt cccaggacat tccactggag gcttggactg catttaggag cccctatccc
      361 ttccctgtcc acactgttag tgagcaattt catatgtttg catttagacc gatagactca
      421 ggacgactca tcacacacac acacagtgta cactgacaca ctcacattcg cacacttagg
      481 tatacagcct gatccttgct ctgacctggt aacaacgctt cctcctcgac tgtgccttct
      541 agaattcgag ctcggtaccc ggggatcctc tagtcagctg acgcgtgcta gcgcggccgc
      601 atcgataagc ttgtcgacga tatctctaga ggatcataat cagccatacc acatttgtag
      661 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      721 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      781 gcatcacaaa tttcacaaat aaagcatttt tt
//
