LOCUS       sequence-150749-         263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bidirectional CAG promoter (compatible with the MXS Chaining Kit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; volume: "11(5):e0155177. doi"; pages: " 
            10.1371/journal.pone.0155177. eCollection 2016"
COMMENT     Sequence Label: MXS_BidirectionalCAG
FEATURES             Location/Qualifiers
     source          1..263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(76..95)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(157..179)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 taggggttcc gcgcacattt ccccgaattg gtcgagaagc ttgtcgacga attctttgcc
       61 aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa
      121 ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc
      181 cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag
      241 agggagaagg ggacggcggc gcg
//
