LOCUS       sequence-150759-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generates B2 inserts suitable for rescue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Wagstaff BJ, Hedges DJ, Derbes RS, Campos Sanchez R, Chiaromonte F, 
            Makova KD, Roy-Engel AM
  TITLE     Rescuing Alu: recovery of new inserts shows LINE-1 preserves Alu 
            activity through A-tail expansion.
  JOURNAL   PLoS Genet. 2012;8(8):e1002842. doi: 10.1371/journal.pgen.1002842. 
            Epub 2012 Aug 9.
  PUBMED    22912586
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012"; volume: "8(8):e1002842. doi"; pages: " 
            10.1371/journal.pgen.1002842. Epub 2012 Aug 9"
COMMENT     Sequence Label: pBS B2rescue A70D
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        101..148
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(229..248)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc ataaagctcc
       61 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
      121 atagtatatc ggcatagtat aatacgacaa ggaggtcgag ccaatatggg atcggccatt
      181 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      241 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      301 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actcgagtac
      361 tccaaaccta atcaatatac tttcgcatac aaattaggtc ccagcggctc cagtgttgca
      421 tcacttcatc cgctagctcc cattaaggag aggtccgact atatcttatg agaagaatac
      481 atcttccccg accgacattt agtctgtgaa ctgcatccat atcaacagaa gatctgttga
      541 cttaggactt ggctgcgtgg ttaggaccat gtccgtcagc ttattaccat accctttgca
      601 aggccatctc aaagtttccc ctgagacttg gtactgaacg gctgttgacc cctttcccgc
      661 aatttgacgg tcttgccttt taaaccgatg caatctattg gtttaaagac tagctaccag
      721 gtgcatgcct gataactttt ccctgcaatt cattatattg ctaattacag gacgaggcag
      781 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
      841 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc g
//
