LOCUS       sequence-150762-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Input plasmid: expresses two recombinases downstream of two 
            inducible promoters. Same as Dual-Recombinase-Controller (plasmid 
            #44456) from Endy Lab except with KanR instead of CamR on the 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR64
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    160..181
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    182..200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    207..225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
       61 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
      121 ggcgccccag ctggcaattc cgacgtcaaa gggcccagag aattcgcggc cgcttctaga
      181 gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg agcacaagct
      241 tgcggctttc acacatagga gctagcgtga gagccctggt agtcatccgc ctgtcccgcg
      301 tcaccgatgc tacgacttca ccggagcgtc agctggagtc ttgccagcag ctctgcgccc
      361 agcgcggctg ggacgtcgtc ggggtagcgg aggatctgga cgtctccggg gcggtcgatc
      421 cgttcgaccg gaagcgcaga ccgaacctgg cccggtggct agcgttcgag gagcaaccgt
      481 ttgacgtgat cgtggcgtac cgggtagatc ggttgacccg atcgatccgg catcttcagc
      541 agctggtcca ctgggccgag gaccacaaga agctggtcgt ctccgcgacc gaagcgcact
      601 tcgatacgac gacgccgttt gcggcggtcg tcatcgcgct tatgggaacg gtggcgcaga
      661 tggaattaga agcgatcaaa gagcggaacc gttcggctgc gcatttcaat atccgcgccg
      721 ggaaataccg aggatccctg ccgccgtggg gatacctgcc tacgcgcgtg gacggggagt
      781 ggcggctggt gccggaccct gtgcagcgag agcgcatcct cgaggtgtat caccgcgtcg
      841 tcgacaacca cgagccgctg catctggtgg cccacgacct gaaccggcgt ggtgtcctgt
      901 cgccgaag
//
