LOCUS       sequence-150763-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Input plasmid: expresses two recombinases downstream of two 
            inducible promoters. Same as Dual-Recombinase-Controller (plasmid 
            #44456) from Endy Lab except with KanR instead of CamR on the 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR64
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctagagtcac acaggaaacc tactagatga aacatcatca ccatcaccac caggccggca
       61 ctaagaaagt agcaatctat acacgagtat ccactactaa ccaagcagag gaaggcttct
      121 caattgatga gcaaattgac cgtttaacaa aatatgctga agcaatgggg tggcaagtat
      181 ctgatactta tactgatgct ggtttttcag gggccaaact tgaacgccca gcaatgcaaa
      241 gattaatcaa cgatatcgag aataaagctt ttgatacagt tcttgtatat aagctagacc
      301 gcctttcacg tagtgtaaga gatactcttt atcttgttaa ggatgtgttc acaaaaaata
      361 aaatagactt tatctcgctt aatgaaagta ttgatacttc ttctgctatg ggtagcttgt
      421 ttctcactat tctttctgca attaatgagt ttgaaagaga gaatataaaa gaacgcatga
      481 ctatgggtaa actagggcga gcgaaatctg gtaagtctat gatgtggact aagacagctt
      541 ttgggtatta ccacaacaga aagacaggta tattagaaat tgttccttta caagctacaa
      601 tagttgaaca aatattcact gattatttat caggaatatc acttacaaaa ttaagagata
      661 aactcaatga atctggacac atcggtaaag atataccgtg gtcttatcgt accctaagac
      721 aaacacttga taatccagtt tactgtggtt atatcaaatt taaggacagc ctatttgaag
      781 gtatgcacaa accaattatc ccttatgaga cttatttaaa agttcaaaaa gagctagaag
      841 aaagac
//
