LOCUS       sequence-150767-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 expressed behind an AHL-inducible construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pLux02
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             51..62
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atagagattg acatccctat cagtgataga gatactgagc actactagag aaagaggaga
       61 aatactagat gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag
      121 cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt
      181 gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa
      241 tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat
      301 atgatcctat agtagattat tctaactcca atcattcacc aattaattgg aatatatttg
      361 aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc
      421 ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt
      481 ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca
      541 taccattaat tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata
      601 aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa
      661 aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt
      721 taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaa
//
