LOCUS       sequence-150779-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression 
            in human pluripotent stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  TITLE     pCAG-SpCas9-U6-gRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-eCas9-GFP-U6-gRNA
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             110..130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagaattcct gaattctgca gatccggtgc caccatggac tataaggacc acgacggaga
       61 ctacaaggat catgatattg attacaaaga cgatgacgat aagatggccc caaagaagaa
      121 gcggaaggtc ggtatccacg gagtcccagc agccgacaag aagtacagca tcggcctgga
      181 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
      241 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
      301 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
      361 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
      421 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
      481 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
      541 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
      601 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
      661 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
      721 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
      781 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
      841 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
      901 caacttcaag agc
//
