LOCUS       sequence-150785-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression 
            in human pluripotent stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     pCAG-SpCas9-U6-gRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-eCas9-GFP-U6-gRNA
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
       61 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
      121 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
      181 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
      241 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
      301 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctgc gctggaaagc gagttcgtgt
      361 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
      421 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
      481 ttaccctggc caacggcgag atccggaagg cgcctctgat cgagacaaac ggcgaaaccg
      541 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
      601 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
      661 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
      721 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
      781 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
      841 aaagaagcag cttcgagaag aatcccatcg acttt
//
