LOCUS       sequence-150787-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Wild-type S. cerevisiae Cus2p gene for recombinant expression and 
            crystallization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Rodgers ML, Tretbar US, Dehaven A, Alwan AA, Luo G, Mast HM, Hoskins
            AA
  TITLE     Conformational dynamics of stem II of the U2 snRNA.
  JOURNAL   RNA. 2015 Dec 2.
  PUBMED    26631165
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2015 
            Dec 2."
COMMENT     Sequence Label: wt Cus2p
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     284..303
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     387..406
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        387..405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    406..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             445..467
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             486..503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             516..536
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattca atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggca
      481 gcagccatca tcatcatcat cacagcagcg gcagagaaaa cttgtatttc cagggccata
      541 tggatgctga tgaattggaa ttgaaaggtc atttgaaaaa attgaaaaaa gaagaattgc
      601 ttagaaggaa acagttaaaa gagagtaatc ttcagaaaag agagcttgaa tataataatg
      661 cctcaaaaaa tacttcaata tatatttctg gtcttcccac agacaaaaca acgaaagaag
      721 gactcactga gcagttttgc aagtatggaa tgataagaac caatcgagat ggggaacccc
      781 tttgcaagct atatgtgaat gataaaggcg ctttcaaagg ggatgccttg atcacttatt
      841 ccaaggaaga aagtgttaca ttagcaatcg aaatgatgaa cgagtcgatc ttcctgggaa
      901 aacagatcag ggtcgaaaga gcccaatttc aaaacaagga aggtgacaat atgcacggga
      961 aagaaaatga tttaaaggag ttcaacggac ctgagcctc
//
