LOCUS       sequence-150789-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Wild-type S. cerevisiae Cus2p gene for recombinant expression and 
            crystallization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Rodgers ML, Tretbar US, Dehaven A, Alwan AA, Luo G, Mast HM, Hoskins
            AA
  TITLE     Conformational dynamics of stem II of the U2 snRNA.
  JOURNAL   RNA. 2015 Dec 2.
  PUBMED    26631165
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2015 
            Dec 2."
COMMENT     Sequence Label: wt Cus2p
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     93..111
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(138..143,144..160,161..166)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(341..389)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
       61 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
      121 ttcaagaatt ctcatgtttg acagcttatc atcgataagc tttaatgcgg tagtttatca
      181 cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc
      241 atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg
      301 ggcctcttgc gggatatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc
      361 gtttagaggc cccaaggggg ttatgctagt tattgctcag gcactagttt acttagttat
      421 taggatccct atataaggtc atcttccact tcgctgtcct cattcttgtc acttgtgctt
      481 gaagtgttct catctccaga aatgaaagca agcaactttt gcccatcaaa ataacgtccg
      541 gtcatgattt tgcaacattg caatgcaact ttattgttct taaagacgac cgtcgcttcc
      601 cccttattgg gagatacaga tatactgtct acttgaccta tctcttcaca gccttccaaa
      661 agatcttcct gaatatcatt tatatcatca tttgtgtatg atttgtaaat attgaatacg
      721 ttggcaaata tcaccgttcg atcagctttt gctaggcttt catcgtcatt gtaatctatt
      781 acttccccct ccccttcaga cttcgctttt tttagcctct ttattggagg ctcaggtccg
      841 ttgaactcct ttaaatcatt ttctttcccg tgcatattgt caccttcc
//
