LOCUS       sequence-150796-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S. cerevisiae Cus2p gene with D282N Mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Rodgers ML, Tretbar US, Dehaven A, Alwan AA, Luo G, Mast HM, Hoskins
            AA
  TITLE     Conformational dynamics of stem II of the U2 snRNA.
  JOURNAL   RNA. 2015 Dec 2.
  PUBMED    26631165
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2015 
            Dec 2."
COMMENT     Sequence Label: D282N Cus2p
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(140..145,146..162,163..168)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(343..391)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
       61 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
      121 tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat
      181 cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg
      241 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc
      301 cgggcctctt gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac
      361 ccgtttagag gccccaaggg ggttatgcta gttattgctc aggcactagt ttacttagtt
      421 attaggatcc ctatataagg tcattttcca cttcgctgtc ctcattcttg tcacttgtgc
      481 ttgaagtgtt ctcatctcca gaaatgaaag caagcaactt ttgcccatca aaataacgtc
      541 cggtcatgat tttgcaacat tgcaatgcaa ctttattgtt cttaaagacg accgtcgctt
      601 cccccttatt gggagataca gatatactgt ctacttgacc tatctcttca cagccttcca
      661 aaagatcttc ctgaatatca tttatatcat catttgtgta tgatttgtaa atattgaata
      721 cgttggcaaa tatcaccgtt cgatcagctt ttgctaggct ttcatcgtca ttgtaatcta
      781 ttacttcccc ctccccttca gacttcgctt tttttagcct ctttattgga ggctcaggtc
      841 cgttgaactc ctttaaa
//
