LOCUS       sequence-150797-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S. cerevisiae Cus2p gene with D282N Mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Rodgers ML, Tretbar US, Dehaven A, Alwan AA, Luo G, Mast HM, Hoskins
            AA
  TITLE     Conformational dynamics of stem II of the U2 snRNA.
  JOURNAL   RNA. 2015 Dec 2.
  PUBMED    26631165
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2015 
            Dec 2."
COMMENT     Sequence Label: D282N Cus2p
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..77)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     283..302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     386..405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        386..404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    405..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             444..466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             485..502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             515..535
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
       61 cgccattcaa tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
      121 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
      181 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      241 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      301 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      361 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
      421 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgggcag
      481 cagccatcat catcatcatc acagcagcgg cagagaaaac ttgtatttcc agggccatat
      541 ggatgctgat gaattggaat tgaaaggtca tttgaaaaaa ttgaaaaaag aagaattgct
      601 tagaaggaaa cagttaaaag agagtaatct tcagaaaaga gagcttgaat ataataatgc
      661 ctcaaaaaat acttcaatat atatttctgg tcttcccaca gacaaaacaa cgaaagaagg
      721 actcactgag cagttttgca agtatggaat gataagaacc aatcgagatg gggaacccct
      781 ttgcaagcta tatgtgaatg ataaaggcgc tttcaaaggg gatgccttga tcacttattc
      841 caaggaagaa agtgttacat tagcaatcga aatgatgaac gagtcgatct tcctgggaaa
      901 acagatcagg gtcgaaagag cccaatttca aaacaaggaa ggtgacaata tgcac
//
