LOCUS       sequence-150803-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis
            genes and UirS sensor kinase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Ramakrishnan P, Tabor JJ
  TITLE     Repurposing Synechocystis PCC6803 UirS-UirR as a UV-violet/green 
            photoreversible transcriptional regulatory tool in E. coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 27.
  PUBMED    27120220
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 27."
COMMENT     Sequence Label: pPR220.08
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             176..187
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(204..238)
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     terminator      complement(355..382)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(398..469)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttcctcttc catggcactg tagacaaagt agagattgcc aaccagctta cggtaggaat
       61 ttttctcgac aacgcccttg aggaagcatt tgacaaagcc gacgttctcc gccatggagt
      121 gggatttttt cgtcccttcc cgcaactggg aagctaagtt gacactcatg gtacctttct
      181 cctctttaat gaattctgtg tgagctagca ttatacctag gactgagcta gctgtcagtt
      241 tttttgacgg taaagccaag ctgaaggctg acaaaatcta tactgttata tccccctttc
      301 ctgggggact taggcggttt aatcgacacg aattatgcag tgatttacta taaacgcaga
      361 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac
      421 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggtct
      481 agattattac taaagtgaaa attccaaccc aaattgagcg ccaccgacat attcatagtg
      541 caattctccc tggagttgtt cagttaggct gtagataagt tgcatgccca aactatctgt
      601 attttccaaa tctatatccc tggaaattcc aactccgtta tcccacacct gtaaactata
      661 gtgactgttc attgaagtga atttaatcga aatttcccct tccgtcgttg ggaaagcatg
      721 ttttaaagca ttggaaacta attcctgaat aattaatccc aaaggaatgg actgctctaa
      781 aggaacttta acttgttcaa ccaacaattt aattttaata ctatcagtat tacagccgta
      841 actttggcag atattgtgag tcaaattttt aagatattga ctaaaatcaa tattagctaa
      901 gtcttcacta cgatataact gc
//
