LOCUS       sequence-151009-         525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 expressed behind an ATc-inducible construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pTet01
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(55..89)
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     misc_feature    90..111
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(113..135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      150..236
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atcactttac ttttatctaa tctggacatc tagtagtcct gtgtgactct agtagctagc
       61 acaataccta ggactgagct agccgtcaac tctagaagcg gccgcgaatt ctcctgtgtg
      121 aaattgttat ccgctttaat taaaggcatc aaataaaacg aaaggctcag tcgaaagact
      181 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      241 cgccctagac agctgggcgc gcccccccta cgggcttgct ctccgggctt cgccctgcgc
      301 ggtcgctgcg ctcccttgcc agcccgtgga tatgtggacg atggccgcga gcggccaccg
      361 gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg gacaggctgc
      421 gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt cgaccacata
      481 cccaccggct ccaactgcgc ggcctgcggc cttgccccat caatt
//
