LOCUS       sequence-151012-         541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 expressed behind an ATc-inducible construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pTet02
FEATURES             Location/Qualifiers
     source          1..541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             36..47
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    92..113
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(115..137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      152..238
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atcactttac ttttatctaa tctggacatc tagtatttct cctctttctc tagtaggcgc
       61 tatcatgcca taccgcaaag gttttgcacc actctagaag cggccgcgaa ttctcctgtg
      121 tgaaattgtt atccgcttta attaaaggca tcaaataaaa cgaaaggctc agtcgaaaga
      181 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      241 gccgccctag acagctgggc gcgccccccc tacgggcttg ctctccgggc ttcgccctgc
      301 gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc gagcggccac
      361 cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga tggacaggct
      421 gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc ttcgaccaca
      481 tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt ttaattttct
      541 c
//
