LOCUS       sequence-151016-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Kapust RB, Tozser J, Fox JD, Anderson DE, Cherry S, Copeland TD, 
            Waugh DS
  TITLE     Tobacco etch virus protease: mechanism of autolysis and rational 
            design of stable mutants with wild-type catalytic proficiency.
  JOURNAL   Protein Eng. 2001 Dec . 14(12):993-1000.
  PUBMED    11809930
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng. 2001 Dec . 14(12):993-1000."
COMMENT     Sequence Label: pRK793
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             60..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             81..788
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
ORIGIN
        1 ataacaataa caacaacctc gggatcgagg gaaggggaga aaatctttat tttcaaggtc
       61 atcatcatca tcatcatcat ggagaaagct tgtttaaggg gccgcgtgat tacaacccga
      121 tatcgagcac catttgtcat ttgacgaatg aatctgatgg gcacacaaca tcgttgtatg
      181 gtattggatt tggtcccttc atcattacaa acaagcactt gtttagaaga aataatggaa
      241 cactgttggt ccaatcacta catggtgtat tcaaggtcaa gaacaccacg actttgcaac
      301 aacacctcat tgatgggagg gacatgataa ttattcgcat gcctaaggat ttcccaccat
      361 ttcctcaaaa gctgaaattt agagagccac aaagggaaga gcgcatatgt cttgtgacaa
      421 ccaacttcca aactaagagc atgtctagca tggtgtcaga cactagttgc acattccctt
      481 catctgatgg catattctgg aagcattgga ttcaaaccaa ggatgggcag tgtggcagtc
      541 cattagtatc aactagagat gggttcattg ttggtataca ctcagcatcg aatttcacca
      601 acacaaacaa ttatttcaca agcgtgccga aaaacttcat ggaattgttg acaaatcagg
      661 aggcgcagca gtgggttagt ggttggcgat taaatgctga ctcagtattg tgggggggcc
      721 ataaagtttt catggtgaaa cctgaagagc cttttcagcc agttaaggaa gcgactcaac
      781 tcatgaatcg tcgtcgccgt cgctaataag gatcctctag agtcgacctg ca
//
