LOCUS       sequence-151018-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE
  TITLE     A UbcH5/ubiquitin noncovalent complex is required for processive 
            BRCA1-directed ubiquitination.
  JOURNAL   Mol Cell. 2006 Mar 17. 21(6):873-80.
  PUBMED    16543155
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Mar 17. 21(6):873-80."
COMMENT     Sequence Label: Ubiquitin WT
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(61..78)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..332)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             339..361
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    376..400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(400..419)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(401..419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(503..522)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        728..805
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gcagccggat cgtcgacgat cctcgagcat atggctgccg cgcggcacca ggccgctgct
       61 gtgatgatga tgatgatggc tgctgcccat ggggtacctc atcaaccacc tcttagtctt
      121 aagacaagat gtaaggtcga ctccttctga atgttgtaat cagacagcgt tctaccgtcc
      181 tcgagctgct taccggcaaa gatcaatctt tgttgatcag gtggaattcc ttccttgtct
      241 tgaattttag ccttaacgtt ttcgatggta tcggatggtt caacttccag agttatggtt
      301 ttaccggtta acgtcttgac gaagatctgc atggatcctc tccttcttaa agttaaacaa
      361 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      421 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
      481 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
      541 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
      601 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
      661 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
      721 gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga
      781 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tc
//
