LOCUS       sequence-151031-        8331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  baculovirus creation plasmid for GST-Plk4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pFastBac GST-Plk4
FEATURES             Location/Qualifiers
     source          1..8331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(589..657,658..1449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(807..826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2109..2128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3920..3937
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4038..4691
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     4649..4671
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             4698..4721
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      6461..6470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7821..7955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7858..7877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7912..7931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  7984..8149
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cagatccatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     4081 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     4141 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     4201 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     4261 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     4321 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     4381 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     4441 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     4501 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     4561 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     4621 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     4681 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc atggcgacct
     4741 gcatcgggga gaagatcgag gattttaaag ttggaaatct gcttggtaaa ggatcatttg
     4801 ctggtgtcta cagagctgag tccattcaca ctggtttgga agttgcaatc aaaatgatag
     4861 ataagaaagc catgtacaaa gcaggaatgg tacagagagt ccaaaatgag gtgaaaatac
     4921 attgccaatt gaaacatcct tctatcttgg agctttataa ctattttgaa gatagcaatt
     4981 atgtgtatct ggtattagaa atgtgccata atggagaaat gaacaggtat ctaaagaata
     5041 gagtgaaacc cttctcagaa aatgaagctc gacacttcat gcaccagatc atcacaggga
     5101 tgttgtatct tcattctcat ggtatactac accgggacct cacactttct aacctcctac
     5161 tgactcgtaa tatgaacatc aagattgctg attttgggct ggcaactcaa ctgaaaatgc
     5221 cacatgaaaa gcactataca ttatgtggaa ctcctaacta catttcacca gaaattgcca
     5281 ctcgaagtgc acatggcctt gaatctgatg tttggtccct gggctgtatg ttttatacat
     5341 tacttatcgg gagaccaccc ttcgacactg acacagtcaa gaacacatta aataaagtag
     5401 tattggcaga ttatgaaatg ccatcttttt tgtcaataga ggccaaggac cttattcacc
     5461 agttacttcg tagaaatcca gcagatcgtt taagtctgtc ttcagtattg gaccatcctt
     5521 ttatgtcccg aaattcttca acaaaaagta aagatttagg aactgtggaa gactcaattg
     5581 atagtgggca tgccacaatt tctactgcaa ttacagcttc ttccagtacc agtataagtg
     5641 gtagtttatt tgacaaaaga agacttttga ttggtcagcc actcccaaat aaaatgactg
     5701 tatttccaaa gaataaaagt tcaactgatt tttcttcttc aggagatgga aacagttttt
     5761 atactcagtg gggaaatcaa gaaaccagta atagtggaag gggaagagta attcaagatg
     5821 cagaagaaag gccacattct cgataccttc gtagagctta ttcctctgat agatctggca
     5881 cttctaatag tcagtctcaa gcaaaaacat atacaatgga acgatgtcac tcagcagaaa
     5941 tgctttcagt gtccaaaaga tcaggaggag gtgaaaatga agagaggtac tcacccacag
     6001 acaacaatgc caacattttt aacttcttta aagaaaagac atccagtagt tctggatctt
     6061 ttgaaagacc tgataacaat caagcactct ccaatcatct ttgtccagga aaaactcctt
     6121 ttccatttgc agacccgaca cctcagactg aaaccgtaca acagtggttt gggaatctgc
     6181 aaataaatgc tcatttaaga aaaactactg aatatgacag catcagccca aaccgggact
     6241 tccagggcca tccagatttg cagaaggaca catcaaaaaa tgcctggact gatacaaaag
     6301 tcaaaaagaa ctctgatgct tctgataatg cacattctgt aaaacagcaa aataccatga
     6361 aatatatgac tgcacttcac agtaaacctg agataatcca acaagaatgt gtttttggct
     6421 cagatcctct ttctgaacag agcaagacta ggggtatgga gccaccatgg ggttatcaga
     6481 atcgtacatt aagaagcatt acatctccgt tggttgctca caggttaaaa ccaatcagac
     6541 agaaaaccaa aaaggctgtg gtgagcatac ttgattcaga ggaggtgtgt gtggagcttg
     6601 taaaggagta tgcatctcaa gaatatgtga aagaagttct tcagatatct agtgatggaa
     6661 atacgatcac tatttattat ccaaatggtg gtagaggttt tcctcttgct gatagaccac
     6721 cctcacctac tgacaacatc agtaggtaca gctttgacaa tttaccagaa aaatactggc
     6781 gaaaatatca atatgcttcc aggtttgtac agcttgtaag atctaaatct cccaaaatca
     6841 cttattttac aagatatgct aaatgcattt tgatggagaa ttctcctggt gctgattttg
     6901 aggtttggtt ttatgatggg gtaaaaatac acaaaacaga agatttcatt caggtgattg
     6961 aaaagacagg gaagtcttac actttaaaaa gtgaaagtga agttaatagc ttgaaagagg
     7021 agataaaaat gtatatggac catgctaatg agggtcatcg tatttgttta gcactggaat
     7081 ccataatttc agaagaggaa aggaaaacta ggagtgctcc ctttttccca ataatcatag
     7141 gaagaaaacc tggtagtact agttcaccta aggccttatc acctcctcct tctgtggatt
     7201 caaattaccc aacgagagag agagcatctt tcaacagaat ggtcatgcat agtgctgctt
     7261 ctccaacaca ggcaccaatc cttaatccct ctatggttac aaatgaagga cttggtctta
     7321 caactacagc ttctggaaca gacatctctt ctaatagtct aaaagattgt cttcctaaat
     7381 cagcacaact tttgaaatct gtttttgtga aaaatgttgg ttgggctaca cagttaacta
     7441 gtggagctgt gtgggttcag tttaatgatg ggtcccagtt ggttgtgcag gcaggagtgt
     7501 cttctatcag ttatacctca ccaaatggtc aaacaactag gtatggagaa aatgaaaaat
     7561 taccagacta catcaaacag aaattacagt gtctgtcttc catccttttg atgttttcta
     7621 atccgactcc taattttcat taagcggccg ctttcgaatc tagagcctgc agtctcgagg
     7681 catgcggtac caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg
     7741 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     7801 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     7861 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7921 ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag cctaggagat
     7981 ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta
     8041 gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa
     8101 aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt
     8161 ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct
     8221 tcggctactt tttctctgtc acagaatgaa aatttttctg tcatctcttc gttattaatg
     8281 tttgtaattg actgaatatc aacgcttatt tgcagcctga atggcgaatg g
//
