LOCUS       sequence-151032-        9282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  baculovirus creation plasmid for GST-STIL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pFastBac GST-STIL
FEATURES             Location/Qualifiers
     source          1..9282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(589..657,658..1449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(807..826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2109..2128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3920..3937
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4038..4691
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     4649..4671
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             4698..4721
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    8772..8906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8809..8828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8863..8882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8935..9100
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cagatccatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     4081 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     4141 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     4201 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     4261 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     4321 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     4381 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     4441 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     4501 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     4561 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     4621 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     4681 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc atggagccta
     4741 tatatccttt tgcacggccc cagatgaata ccaggtttcc ttcaagcagg atggtacctt
     4801 tccactttcc tccatcaaaa tgtgcacttt ggaacccaac gccaactgga gatttcatct
     4861 acttacatct cagttactac agaaatccaa agcttgtggt gactgagaag accatccgac
     4921 ttgcttatcg tcatgctaag cagaataaaa aaaattcgtc atgcttttta cttggttctc
     4981 tgacagcaga cgaagatgaa gaaggtgtaa cattgacagt agatcgcttt gatcctggtc
     5041 gagaagtacc tgaatgccta gaaataaccc ctactgcttc tcttcctggg gactttttga
     5101 ttccatgcaa agttcatact caagaacttt gttcaagaga aatgatagtt cacagtgtag
     5161 atgacttcag ttcagcttta aaggctctac agtgccatat atgtagcaaa gattccttgg
     5221 actgtggtaa gctgctttcc ctaagagttc atatcacttc cagggagagt ttggacagtg
     5281 tggaatttga cttgcattgg gcagcagtaa ctctagcaaa taactttaaa tgcacacctg
     5341 tgaagcccat ccccattatt ccaacagctc tggcaagaaa cttgagcagt aatctgaata
     5401 tttctcaagt tcaagggact tataaatatg gatatcttac catggatgaa acacgcaaat
     5461 tgttactttt gttggaatct gatcccaagg tttattctct accattggtg ggaatttggc
     5521 tgtctggaat tacacatatc tatagtcctc aggtatgggc ttgctgtttg cgatacatat
     5581 tcaattcttc tgttcaagaa agggtttttt cagaatctgg aaatttcatc atagttctct
     5641 attctatgac acataaggaa cctgagtttt atgaatgctt cccttgtgat ggcaagatac
     5701 ctgactttcg gtttcagttg ctaaccagta aggaaacatt acatcttttc aaaaatgttg
     5761 aacctcctga caaaaatcca atccgttgtg aactgagcgc tgaaagccaa aatgcagaaa
     5821 cagagttttt cagtaaggct tccaagaatt tttcaattaa gaggtcttcc caaaagttat
     5881 cttctgggaa gatgccaata catgatcacg actctggtgt tgaagatgaa gatttttctc
     5941 caagaccaat tcctagtcct catccagtga gtcagaagat ttctaagatc caaccatcag
     6001 ttcctgaact ttcacttgtg ttggatggca atttcataga atcaaaccct ctgcctactc
     6061 cattggaaat ggtgaataat gaaaatcctc ctttgattaa ccacttggaa cacttgaagc
     6121 cattgcaacc ccagctttat gatgagaaac acagtccaga agttgaagct ggagagcctt
     6181 ccttgagagg aataccaaat cagttaaacc aggataaacc agctcttttg agacactgca
     6241 aagtaagaca gccacctgcc tataagaaag ggaaccccca taccaggaac agtattaaac
     6301 catcttctca taatgggcca tctcatgata tatttgaaaa actccaaacc gtgtctgccg
     6361 gaaatgtaca aaacgaagag tatcctataa gaccctccac acttaattct aggcagtctt
     6421 ctcttgcccc gcagtcccaa ccacacgatt ttgttttttc accccataat tcaggaagac
     6481 caatggaact tcagatacct actcccccac tgccatctta ctgttccaca aacgtttgca
     6541 ggtgttgtca gcatcatagt catattcaat atagtccgct aaattcttgg caaggagcaa
     6601 acacagttgg ctccattcaa gatgtccagt ctgaagccct tcaaaagcat tcattatttc
     6661 acccaagtgg atgtccagcc ctgtactgta atgcattctg ttcttcaagt agtcctatag
     6721 ccttgagacc tcagggagat atgggcagtt gttctcccca cagcaatatt gaaccatcgc
     6781 ctgtggcaag accgccttca catatggact tatgtaaccc acagccttgc acagtgtgca
     6841 tgcacacacc caagactgag tcagataatg gaatgatggg actatctcca gatgcatatc
     6901 ggttcctcac agaacaagac agacagctaa gactacttca ggcacagatt cagcgtttgt
     6961 tggaagcaca gtctctgatg ccctgttccc ctaagacaac tgctgttgaa gacacagtgc
     7021 aagctggaag acaaatggag ttggtttctg tggaagcaca gtcttcccct ggcttgcaca
     7081 tgagaaaagg tgtaagcatt gctgtgagca caggtgctag cttgttttgg aatgcagcag
     7141 gtgaggatca agagcctgac tctcaaatga agcaagatga taccaaaatt tccagtgagg
     7201 acatgaattt ttctgtcgat attaataatg aagtcacaag tcttccaggt agtgcatctt
     7261 cattaaaagc agttgatatt cccagttttg aagagagcaa cattgctgtg gaagaagaat
     7321 ttaaccagcc actttctgta tccaactctt ctctagttgt gagaaaagaa cctgatgtac
     7381 ctgtgttctt tccaagtggc cagctggcag aaagtgtaag catgtgttta cagactggac
     7441 caacaggggg tgccagtaac aattctgaaa catcagagga accaaaaatt gagcatgtaa
     7501 tgcaaccctt gcttcatcaa ccatcagata accagaaaat ttaccaggat ttattgggtc
     7561 aagtaaacca cctattaaat agttcctcca aggaaactga gcagccgtct accaaagcag
     7621 taattatcag tcatgaatgc accagaaccc aaaacgttta ccatacaaag aaaaaaacac
     7681 atcattcaag actggtggac aaagattgtg tccttaatgc aactcttaag caactaagaa
     7741 gccttggagt aaaaattgat tctcccacta aagtgaagaa aaatgcacat aacgtggatc
     7801 acgccagtgt gttggcatgc atcagcccag aagcagtgat ctctggatta aactgcatgt
     7861 catttgctaa tgttggcatg agcggcttaa gccccaatgg tgtggatttg agcatggagg
     7921 caaatgctat agctctgaaa tatttaaatg aaaatcagct gtcacaactg tctgtcactc
     7981 gatcgaacca aaataattgt gacccattca gccttctcca tattaataca gacagaagca
     8041 cagtggggct tagtttaatt tcaccaaaca acatgtcatt tgcaaccaaa aaatatatga
     8101 agagatatgg actcctacaa agcagtgaca atagtgaaga tgaagaggaa cctcccgaca
     8161 atgcagatag caagagtgaa tatttattga atcagaacct taggtccata cccgaacagc
     8221 ttggtggtca gaaagagcct tctaagaatg accatgaaat aattaattgt tctaactgtg
     8281 aatctgtggg gaccaacgca gatacgccag tattgagaaa tattacaaat gaagttttgc
     8341 agacaaaagc aaaacagcag ttgactgaaa agccagcttt cttagtaaag aaccttaaac
     8401 caagtcctgc agtgaacctt cgaaccggga aagcagagtt cactcaacat cctgagaaag
     8461 aaaatgaagg ggacattaca atttttcctg aaagtttgca accttctgaa acgctaaagc
     8521 agatgaatag catgaattca gtaggcacct tcttagatgt aaaacgtctc agacagttac
     8581 caaaattatt ttaagcggcc gctttcgaat ctagagcctg cagtctcgag gcatgcggta
     8641 ccaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
     8701 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     8761 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8821 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8881 tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctaggaga tccgaaccag
     8941 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     9001 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     9061 ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg
     9121 ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact
     9181 ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat gtttgtaatt
     9241 gactgaatat caacgcttat ttgcagcctg aatggcgaat gg
//
