LOCUS       sequence-151034-        9906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression plasmid for MycLAP-STIL coiled-coil deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO MycLAP-STIL ∆CC
FEATURES             Location/Qualifiers
     source          1..9906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1154..1183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1187..1189,1190..1900)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1187..1189,1190..1900)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1229..1250)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1490..1509)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1837..1858
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1946..1966
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1979..2023
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(5858..5875)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5864..6088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6221..6240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6372..6419
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6427..7449
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6427..7449
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7579..7700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7616..7635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7670..7689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7749..7765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7749..7765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7762..7784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7773..7789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7797..7803,7804..7821,7822..7827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7842..7863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7980..7997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8151..8739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8231..8250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8910..9701,9702..9770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9533..9552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9771..9875)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccc cggggaattc ggcagatccc gaggtccgac
     1021 agcgcccggc ccagatcccc acgcctgcca ggagcaagcc gagagccagc cggccggcgc
     1081 actccgactc cgagcagtct ctgtccttcg acccgagccc cgcgcccttt ccgggacccc
     1141 tgccccgcgg atggagcaga agctcatctc agaagaagac ctcgacgtga gcaagggcga
     1201 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1261 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1321 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1381 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1441 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1501 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1561 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1621 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1681 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1741 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1801 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1861 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggagggg atcggtggag
     1921 ctccaccggt ggcggacgct ctagagagaa tctttatttt cagggcgccg ccaaattcaa
     1981 agaaaccgct gctgctaaat tcgaacgcca gcacatggac agcggaggtg gatccactag
     2041 tctcgagcct atatatcctt ttgcacggcc ccagatgaat accaggtttc cttcaagcag
     2101 gatggtacct ttccactttc ctccatcaaa atgtgcactt tggaacccaa cgccaactgg
     2161 agatttcatc tacttacatc tcagttacta cagaaatcca aagcttgtgg tgactgagaa
     2221 gaccatccga cttgcttatc gtcatgctaa gcagaataaa aaaaattcgt catgcttttt
     2281 acttggttct ctgacagcag acgaagatga agaaggtgta acattgacag tagatcgctt
     2341 tgatcctggt cgagaagtac ctgaatgcct agaaataacc cctactgctt ctcttcctgg
     2401 ggactttttg attccatgca aagttcatac tcaagaactt tgttcaagag aaatgatagt
     2461 tcacagtgta gatgacttca gttcagcttt aaaggctcta cagtgccata tatgtagcaa
     2521 agattccttg gactgtggta agctgctttc cctaagagtt catatcactt ccagggagag
     2581 tttggacagt gtggaatttg acttgcattg ggcagcagta actctagcaa ataactttaa
     2641 atgcacacct gtgaagccca tccccattat tccaacagct ctggcaagaa acttgagcag
     2701 taatctgaat atttctcaag ttcaagggac ttataaatat ggatatctta ccatggatga
     2761 aacacgcaaa ttgttacttt tgttggaatc tgatcccaag gtttattctc taccattggt
     2821 gggaatttgg ctgtctggaa ttacacatat ctatagtcct caggtatggg cttgctgttt
     2881 gcgatacata ttcaattctt ctgttcaaga aagggttttt tcagaatctg gaaatttcat
     2941 catagttctc tattctatga cacataagga acctgagttt tatgaatgct tcccttgtga
     3001 tggcaagata cctgactttc ggtttcagtt gctaaccagt aaggaaacat tacatctttt
     3061 caaaaatgtt gaacctcctg acaaaaatcc aatccgttgt gaactgagcg ctgaaagcca
     3121 aaatgcagaa acagagtttt tcagtaaggc ttccaagaat ttttcaatta agaggtcttc
     3181 ccaaaagtta tcttctggga agatgccaat acatgatcac gactctggtg ttgaagatga
     3241 agatttttct ccaagaccaa ttcctagtcc tcatccagtg agtcagaaga tttctaagat
     3301 ccaaccatca gttcctgaac tttcacttgt gttggatggc aatttcatag aatcaaaccc
     3361 tctgcctact ccattggaaa tggtgaataa tgaaaatcct cctttgatta accacttgga
     3421 acacttgaag ccattgcaac cccagcttta tgatgagaaa cacagtccag aagttgaagc
     3481 tggagagcct tccttgagag gaataccaaa tcagttaaac caggataaac cagctctttt
     3541 gagacactgc aaagtaagac agccacctgc ctataagaaa gggaaccccc ataccaggaa
     3601 cagtattaaa ccatcttctc ataatgggcc atctcatgat atatttgaaa aactccaaac
     3661 cgtgtctgcc ggaaatgtac aaaacgaaga gtatcctata agaccctcca cacttaattc
     3721 taggcagtct tctcttgccc cgcagtccca accacacgat tttgtttttt caccccataa
     3781 ttcaggaaga ccaatggaac ttcagatacc tactccccca ctgccatctt actgttccac
     3841 aaacgtttgc aggtgttgtc agcatcatag tcatattcaa tatagtccgc taaattcttg
     3901 gcaaggagca aacacagttg gctccattca agatgtccag tctgaagccc ttcaaaagca
     3961 ttcattattt cacccaagtg gatgtccagc cctgtactgt aatgcattct gttcttcaag
     4021 tagtcctata gccttgagac ctcagggaga tatgggcagt tgttctcccc acagcaatat
     4081 tgaaccatcg cctgtggcaa gaccgccttc acatatggac ttatgtaacc cacagccttg
     4141 cacagtgtgc atgcacacac ccaagactga gtcagataat ggaatgatgg gactatctcc
     4201 atctctgatg ccctgttccc ctaagacaac tgctgttgaa gacacagtgc aagctggaag
     4261 acaaatggag ttggtttctg tggaagcaca gtcttcccct ggcttgcaca tgagaaaagg
     4321 tgtaagcatt gctgtgagca caggtgctag cttgttttgg aatgcagcag gtgaggatca
     4381 agagcctgac tctcaaatga agcaagatga taccaaaatt tccagtgagg acatgaattt
     4441 ttctgtcgat attaataatg aagtcacaag tcttccaggt agtgcatctt cattaaaagc
     4501 agttgatatt cccagttttg aagagagcaa cattgctgtg gaagaagaat ttaaccagcc
     4561 actttctgta tccaactctt ctctagttgt gagaaaagaa cctgatgtac ctgtgttctt
     4621 tccaagtggc cagctggcag aaagtgtaag catgtgttta cagactggac caacaggggg
     4681 tgccagtaac aattctgaaa catcagagga accaaaaatt gagcatgtaa tgcaaccctt
     4741 gcttcatcaa ccatcagata accagaaaat ttaccaggat ttattgggtc aagtaaacca
     4801 cctattaaat agttcctcca aggaaactga gcagccgtct accaaagcag taattatcag
     4861 tcatgaatgc accagaaccc aaaacgttta ccatacaaag aaaaaaacac atcattcaag
     4921 actggtggac aaagattgtg tccttaatgc aactcttaag caactaagaa gccttggagt
     4981 aaaaattgat tctcccacta aagtgaagaa aaatgcacat aacgtggatc acgccagtgt
     5041 gttggcatgc atcagcccag aagcagtgat ctctggatta aactgcatgt catttgctaa
     5101 tgttggcatg agcggcttaa gccccaatgg tgtggatttg agcatggagg caaatgctat
     5161 agctctgaaa tatttaaatg aaaatcagct gtcacaactg tctgtcactc gatcgaacca
     5221 aaataattgt gacccattca gccttctcca tattaataca gacagaagca cagtggggct
     5281 tagtttaatt tcaccaaaca acatgtcatt tgcaaccaaa aaatatatga agagatatgg
     5341 actcctacaa agcagtgaca atagtgaaga tgaagaggaa cctcccgaca atgcagatag
     5401 caagagtgaa tatttattga atcagaacct taggtccata cccgaacagc ttggtggtca
     5461 gaaagagcct tctaagaatg accatgaaat aattaattgt tctaactgtg aatctgtggg
     5521 gaccaacgca gatacgccag tattgagaaa tattacaaat gaagttttgc agacaaaagc
     5581 aaaacagcag ttgactgaaa agccagcttt cttagtaaag aaccttaaac caagtcctgc
     5641 agtgaacctt cgaaccggga aagcagagtt cactcaacat cctgagaaag aaaatgaagg
     5701 ggacattaca atttttcctg aaagtttgca accttctgaa acgctaaagc agatgaatag
     5761 catgaattca gtaggcacct tcttagatgt aaaacgtctc agacagttac caaaattatt
     5821 ttaagcggcc gcgggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5881 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5941 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6001 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6061 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     6121 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6181 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6241 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6301 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6361 tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc
     6421 ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     6481 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     6541 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     6601 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     6661 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     6721 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     6781 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     6841 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     6901 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     6961 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     7021 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     7081 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     7141 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     7201 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     7261 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     7321 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     7381 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     7441 aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     7501 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     7561 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7621 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7681 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7741 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7801 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7861 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7921 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7981 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8041 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8101 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8161 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8221 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8281 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8341 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8401 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8461 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8521 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8581 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8641 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8701 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8761 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8821 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8881 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8941 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9001 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9061 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9121 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9181 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9241 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9301 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9361 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9421 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9481 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9541 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9601 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9661 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9721 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9781 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9841 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9901 gacgtc
//
