LOCUS       sequence-151041-        8645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression plasmid for Plk4 with multi-phospho degron 
            deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO Plk4 ∆24-mCherry
FEATURES             Location/Qualifiers
     source          1..8645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      993..1002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2657..2666
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3852..4562
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3997..4015)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4265..4284
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4597..4614)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4603..4827
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4960..4979)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5111..5158
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5166..6188
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5166..6188
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6318..6439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6355..6374)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6409..6428
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6488..6504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6488..6504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6501..6523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6512..6528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6536..6542,6543..6560,6561..6566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6581..6602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6719..6736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6890..7478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6970..6989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7649..8440,8441..8509))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8272..8291
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8510..8614)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttaccggtg ccgccaccat ggcgacctgc atcggggaga
     1021 agatcgagga ttttaaagtt ggaaatctgc ttggtaaagg atcatttgct ggtgtctaca
     1081 gagctgagtc cattcacact ggtttggaag ttgcaatcaa aatgatagat aagaaagcca
     1141 tgtacaaagc aggaatggta cagagagtcc aaaatgaggt gaaaatacat tgccaattga
     1201 aacatccttc tatcttggag ctttataact attttgaaga tagcaattat gtgtatctgg
     1261 tattagaaat gtgccataat ggagaaatga acaggtatct aaagaataga gtgaaaccct
     1321 tctcagaaaa tgaagctcga cacttcatgc accagatcat cacagggatg ttgtatcttc
     1381 attctcatgg tatactacac cgggacctca cactttctaa cctcctactg actcgtaata
     1441 tgaacatcaa gattgctgat tttgggctgg caactcaact gaaaatgcca catgaaaagc
     1501 actatacatt atgtggaact cctaactaca tttcaccaga aattgccact cgaagtgcac
     1561 atggccttga atctgatgtt tggtccctgg gctgtatgtt ttatacatta cttatcggga
     1621 gaccaccctt cgacactgac acagtcaaga acacattaaa taaagtagta ttggcagatt
     1681 atgaaatgcc atcttttttg tcaatagagg ccaaggacct tattcaccag ttacttcgta
     1741 gaaatccagc agatcgttta agtctgtctt cagtattgga ccatcctttt atgtcccgaa
     1801 attcttcaac aaaaagtaaa gatttaggaa ctgtggaaga cttatttgac aaaagaagac
     1861 ttttgattgg tcagccactc ccaaataaaa tgactgtatt tccaaagaat aaaagttcaa
     1921 ctgatttttc ttcttcagga gatggaaaca gtttttatac tcagtgggga aatcaagaaa
     1981 ccagtaatag tggaagggga agagtaattc aagatgcaga agaaaggcca cattctcgat
     2041 accttcgtag agcttattcc tctgatagat ctggcacttc taatagtcag tctcaagcaa
     2101 aaacatatac aatggaacga tgtcactcag cagaaatgct ttcagtgtcc aaaagatcag
     2161 gaggaggtga aaatgaagag aggtactcac ccacagacaa caatgccaac atttttaact
     2221 tctttaaaga aaagacatcc agtagttctg gatcttttga aagacctgat aacaatcaag
     2281 cactctccaa tcatctttgt ccaggaaaaa ctccttttcc atttgcagac ccgacacctc
     2341 agactgaaac cgtacaacag tggtttggga atctgcaaat aaatgctcat ttaagaaaaa
     2401 ctactgaata tgacagcatc agcccaaacc gggacttcca gggccatcca gatttgcaga
     2461 aggacacatc aaaaaatgcc tggactgata caaaagtcaa aaagaactct gatgcttctg
     2521 ataatgcaca ttctgtaaaa cagcaaaata ccatgaaata tatgactgca cttcacagta
     2581 aacctgagat aatccaacaa gaatgtgttt ttggctcaga tcctctttct gaacagagca
     2641 agactagggg tatggagcca ccatggggtt atcagaatcg tacattaaga agcattacat
     2701 ctccgttggt tgctcacagg ttaaaaccaa tcagacagaa aaccaaaaag gctgtggtga
     2761 gcatacttga ttcagaggag gtgtgtgtgg agcttgtaaa ggagtatgca tctcaagaat
     2821 atgtgaaaga agttcttcag atatctagtg atggaaatac gatcactatt tattatccaa
     2881 atggtggtag aggttttcct cttgctgata gaccaccctc acctactgac aacatcagta
     2941 ggtacagctt tgacaattta ccagaaaaat actggcgaaa atatcaatat gcttccaggt
     3001 ttgtacagct tgtaagatct aaatctccca aaatcactta ttttacaaga tatgctaaat
     3061 gcattttgat ggagaattct cctggtgctg attttgaggt ttggttttat gatggggtaa
     3121 aaatacacaa aacagaagat ttcattcagg tgattgaaaa gacagggaag tcttacactt
     3181 taaaaagtga aagtgaagtt aatagcttga aagaggagat aaaaatgtat atggaccatg
     3241 ctaatgaggg tcatcgtatt tgtttagcac tggaatccat aatttcagaa gaggaaagga
     3301 aaactaggag tgctcccttt ttcccaataa tcataggaag aaaacctggt agtactagtt
     3361 cacctaaggc cttatcacct cctccttctg tggattcaaa ttacccaacg agagagagag
     3421 catctttcaa cagaatggtc atgcatagtg ctgcttctcc aacacaggca ccaatcctta
     3481 atccctctat ggttacaaat gaaggacttg gtcttacaac tacagcttct ggaacagaca
     3541 tctcttctaa tagtctaaaa gattgtcttc ctaaatcagc acaacttttg aaatctgttt
     3601 ttgtgaaaaa tgttggttgg gctacacagt taactagtgg agctgtgtgg gttcagttta
     3661 atgatgggtc ccagttggtt gtgcaggcag gagtgtcttc tatcagttat acctcaccaa
     3721 atggtcaaac aactaggtat ggagaaaatg aaaaattacc agactacatc aaacagaaat
     3781 tacagtgtct gtcttccatc cttttgatgt tttctaatcc gactcctaat tttcatcccg
     3841 gggcggatcc aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     3901 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     3961 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     4021 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     4081 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     4141 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     4201 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     4261 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     4321 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     4381 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     4441 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     4501 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     4561 aaagcggccg cgggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     4621 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4681 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4741 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4801 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4861 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4921 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4981 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5041 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5101 cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct
     5161 tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     5221 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     5281 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     5341 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     5401 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     5461 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     5521 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     5581 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     5641 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     5701 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     5761 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     5821 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     5881 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     5941 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     6001 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     6061 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     6121 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     6181 aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6241 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6301 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6361 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6421 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6481 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6541 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6601 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6661 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7321 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8641 acgtc
//
