LOCUS       sequence-151053-       10186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and 
            gRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pHACK-GAL4>QF2
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1244..1277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    1291..1324
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1493..1526
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             1566..2003
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2760..2813
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(4146..4179)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4243..4364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4254..4273)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4308..4327
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4484..5194)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     5031..5049
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(5372..5405)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6440..6784
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    6794..6827
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        6843..7639
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        7659..7734
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7735..8155
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        8176..8251
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(8684..8717)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(9187..9204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9187..9203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9302..9319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9473..10061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9553..9572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10185..10186,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggcgg gtgccagggc gtgcccttgg gctccccggg
     1321 cgcgtacaag ccgaaggcgc gcatgatgaa ataacataag gtggtcccgt cgaaagccga
     1381 agcttaccga agtatacact taaattcagt gcacgtttgc ttgttgagag gaaaggttgt
     1441 gtgcggacga attttttttt gaaaacatta acccttacgt aacgcgagat ctgaagttcc
     1501 tattctctag aaagtatagg aacttctagg gataacaggg taatagatct ggatcctaat
     1561 gatgaaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca
     1621 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct
     1681 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa
     1741 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga
     1801 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag
     1861 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc
     1921 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag
     1981 gtcaaagaca gttgactgta tcgattgact cggcagctca tcatgataac tccacaattc
     2041 cgttggattt tatgcccagg gatgctcttc atggatttga ttggtctgaa gaggatgaca
     2101 tgtcggatgg cttgcccttc ctgaaaacgg accccaacaa taatgggttc tttggcgacg
     2161 gttctctctt atgtattctt cgatctattg gctttaaacc ggaaaattac acgaactcta
     2221 acgttaacag gctcccgacc atgattacgg atagatacac gttggcttct agatccacaa
     2281 catcccgttt acttcaaagt tatctcaata attttcaccc ctactgccct atcgtgcact
     2341 caccgacgct aatgatgttg tataataacc agattgaaat cgcgtcgaag gatcaatggc
     2401 aaatcctttt taactgcata ttagccattg gagcctggtg tatagagggg gaatctactg
     2461 atatagatgt tttttactat caaaatgcta aatctcattt gacgagcaag gtcttcgagt
     2521 caggttccat aattttggtg acagccctac atcttctgtc gcgatataca cagtggaggc
     2581 agaaaacaaa tactagctat aattttcaca gcttttccat aagaatggcc atatcattgg
     2641 gcttgaatag ggacctcccc tcgtccttca gtgatagcag cattctggaa caaagacgcc
     2701 gaatttggtg gtctgtctac tcttgggaga tccaattgtc cctgcttgga tcctcgcgtg
     2761 agggccgcgg cagcctgctg acctgcggcg atgtggagga gaaccccggg ccccctagga
     2821 tgccacccaa gcgcaaaacg cttaacgctg cggctgaggc taacgctcat gccgacggac
     2881 acgccgacgg aaacgccgac ggacacgtgg ccaatacggc cgcgtcctcg aataatgcga
     2941 ggttcgctga tctcactaac atcgatactc cgggtctggg acccacaact acgaccctgc
     3001 tcgtggaacc agcacgctca aagcgtcaac gagtgtcccg cgcatgcgac cagtgccgtg
     3061 cagcccgaga gaaatgcgac ggaatacagc ctgcgtgttt cccgtgcgtt tcccagggaa
     3121 ggtcctgcac ttatcaggct tcgccgaaaa agaggggagt tcaaaccggt tatattcgta
     3181 cgctggagct cgccctcgcc tggatgtttg aaaatgtcgc gcgttccgaa gatgccttgc
     3241 ataacctcct cgtccgtgac gccggacaag gatcagctct gctcgttggt aaagattcgc
     3301 cggctgccga gcgactccat gcccgttggg ctactagccg tgtcaataag agcattaccc
     3361 gcctcctccg tcagttggag ctccctccta ccgccacggc tacggcctcg ataatgccgc
     3421 acgtgatgga gcagcctctc agtaccagca ttaaccccgt caacgaccgc ttcaacggta
     3481 ttcccaaccc cactccgtat aactccgatg cagctctcga tgctatcact cagaccaacg
     3541 attatggaag cgtaaataca catggtatcc tctctactta cccgccaccg gctacgcacc
     3601 ttaatgaagc ttccgtcgct ctcgctcccg gtggcgcccc cccccgaccg cctcctccgt
     3661 atgttgacag cacgaccaat cacccgccgt accactcgaa tctggttcca atggcgaact
     3721 ttggttactc gaccgttgat tacgatgcca tggttgacga tttggctagc attgaataca
     3781 cggacgctgt ggatgtcgac ccacagttta tgaccaatct gggattcgtt cctggatgta
     3841 acttctccga cattaataca tacgaacagt gatgacgtct atcgataccg tcgactaaag
     3901 ccaaatagaa aattattcag ttcctggctt aagtttttaa aagtgatatt atttatttgg
     3961 ttgtaaccaa ccaaaagaat gtaaataact aatacataat tatgttagtt ttaagttagc
     4021 aacaaattga ttttagctat attagctact tggttaataa atagaatata tttatttaaa
     4081 gataattgcg tttttattgt cagggagtga gtttgcttaa aaactcgttt agatccaccg
     4141 catgcataac ttcgtatagc atacattata cgaagttatg gtaccactcg acgattaacc
     4201 gttagtttag caagtataat tgaatttttt ataagatcca gctaagatac attgatgagt
     4261 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     4321 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     4381 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     4441 tctacaaatg tggtatggct gattatgatc agagtcgcgg cgcttacttg tacagctcgt
     4501 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
     4561 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
     4621 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
     4681 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
     4741 aggaggcctc ccagcccatg gttttcttct gcattacggg gccgtcggag gggaagttgg
     4801 tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca
     4861 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     4921 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
     4981 acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct
     5041 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     5101 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
     5161 tggccatgtt atcctcctcg cccttgctca ccatgggccg attgtttagc ttgttcagct
     5221 gcgcttgttt atttgcttag ctttcgctta gcgacgtgtt cactttgctt gtttgaattg
     5281 aattgtcgct ccgtagacga agcgcctcta tttatactcc ggcgctaatt gaattagctc
     5341 taattgaatt agtctctaat tgaatgacgt cataacttcg tatagcatac attatacgaa
     5401 gttatagatc actagtcatg gcatcattga aacagcaagg ctcttacaag ttttcacaaa
     5461 aatctatgaa ctagacaaaa cagtaactgc agaaaaaagt cctatatgtg caaaaaaatg
     5521 cttgatgatt tgtaatgaga ttgaggaggt ttcgagacag gcaccaaagt ttttacaaat
     5581 ggatatttcc accaccgctc taaccaattt gttgaaggaa cacccttggc tatcctttac
     5641 aagattcgaa ctgaagtgga aacagttgtc tcttatcatt tatgtattaa gagatttttt
     5701 cactaatttt acccagaaaa agtcacaact agaacaggat caaaatgatc atcaaagtta
     5761 tgaagttaaa cgatgctcca tcatgttaag cgatgcagca caaagaactg ttatgtctgt
     5821 aagtagctat atggacaatc ataatgtcac cccatatttt gcctggaatt gttcttatta
     5881 cttgttcaat gcagtcctag tacccataaa gactctactc tcaaactcaa aatcgaatgc
     5941 tgagaataac gagaccgcac aattattaca acaaattaac actgttctga tgctattaaa
     6001 aaaactggcc acttttaaaa tccagacttg tgaaaaatac attcaagtac tggaagaggt
     6061 atgtgcgccg tttctgttat cacagtgtgc aatcccatta ccgcatatca gttataacaa
     6121 tagtaatggt agcgccatta aaaatattgt cggttctgca actatcgccc aataccctac
     6181 tcttccggag gaaaatgtca acaatatcag tgttaaatat gtttctcctg gctcagtagg
     6241 gccttcacct gtgccattga aatcaggagc aagtttcagt gatctagtca agctgttatc
     6301 taaccgtcca ccctctcgta actctccagt gacaatacca agaagcacac cttcgcatcg
     6361 ctcagtcacg ccttttctag ggcaacagca acagctgcaa tcattagtgc cactgacccc
     6421 gtctgctttg tttggtggcg ccaattttaa tcaaagtggg aatattgctg atagctcatt
     6481 gtccttcact ttcactaaca gtagcaacgg tccgaacctc ataacaactc aaacaaattc
     6541 tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat gataacttca tgaataatga
     6601 aatcacggct agtaaaattg atgatggtaa taattcaaaa ccactgtcac ctggttggac
     6661 ggaccaaact gcgtataacg cgtttggaat cactacaggg atgtttaata ccactacaat
     6721 ggatgatgta tataactatc tattcgatga tgaagatacc ccaccaaacc caaaaaaaga
     6781 gtaatagact agtgaagttc ctattctcta gaaagtatag gaacttctag tgatctgaat
     6841 tcattttcaa cgtcctcgat agtatagtgg ttagtatccc cgcctgtcac gcgggagacc
     6901 ggggttcaat tccccgtcgg ggagaatctg tgattctttt tttttttctt ttactttgtt
     6961 atataaacaa tttttgtttt aattgaatct aatttgccat tgcttttagg aatctcaggc
     7021 atccagcaag cgtttgtccg ccgaatcgcc catcagtgaa gaagatcctg tggcggctac
     7081 gaaaatctcc ccggccatgt cggcctccac ctccagcgaa aaacccatca gcgagctggc
     7141 cacctctgtg ctgacccacc gctttccaga ctccacctcc tcacccggcg aacatggcct
     7201 tggacgaatg cagttgtcga tccgctacag cgcccagcgt caaaaactag acgtgaccat
     7261 acacaaaatc cagaagatac cacttcgcga tcccagcaat atccccgatc cgtatgttaa
     7321 gctgtatctg ttgcctggac gcaccaagga gtcgaaacgc aagacgagcg tgatcaagga
     7381 caactgcaac cccgtctacg atgcatcctt tgagtacctg atttccattg ccgaactcag
     7441 gcagacggaa ctggaggtga cggtgtgcac ccaaaaggga ttcctatccg gcggtagtcc
     7501 catcattggc atggtaggta cccgaaagca accccttagt tacagacaca gcgcgtacgt
     7561 ccttcgcatc cttatgattc ccaagtacat attctgcaag agtacagtat atataggaaa
     7621 gatatccggg tgaacttcga tgtgcagcgt accacaacgt tttagagcta gaaatagcaa
     7681 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     7741 gctcacctgt gattgctcct actcaaatac aaaaacatca aattttctgt caataaagca
     7801 tatttattta tatttatttt acaggaaaga attcctttta aagtgtattt taacctataa
     7861 tgaaaaacga ttaaaaaaaa tacataaaat aattcgaaaa tttttgaata gcccaggttg
     7921 ataaaaattc atttcatacg ttttataact tatgccccta agtatttttt gaccatagtg
     7981 tttcaattct acattaattt tacagagtag aatgaaacgc cacctactca gccaagaggc
     8041 gaaaaggtta gctcgccaag cagagagggc gccagtgctc actacttttt ataattctca
     8101 acttcttttt ccagactcag ttcgtatata tagacctatt ttcaatttaa cgtcgtgtat
     8161 tctgagaaag ctggagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     8221 tcaacttgaa aaagtggcac cgagtcggtg cttttttgcc tacctggagc ctgagagttg
     8281 ttcaataaaa taaaaatgtt tcgttttttt gctttcgcca gtatttatta tttttcatca
     8341 atatgtattc aatttggtat gtatttagta attgtaatat atagacaatg gttttccgtt
     8401 gacgtacata catctgacgt gtgtttattt agacataata gttatgtttt cacatctttt
     8461 taatgttcgc ttaatgcgta tgcttaatta attctgacgt aagctagtta caaaccccac
     8521 ggacatgcta agggttaatc aacaatcata tcgctgtctc actcagactc aatacgacac
     8581 tcagaatact attcctttca ctcgcactta ttgcaagcat acgttaagtg gatgtctctt
     8641 gccgacggga ccaccttatg ttatttcatc atgaaaatga agtgaagttc ctatactttc
     8701 tagagaatag gaacttctat agtgagtcga ataagggcga cacaaaattt attctaaatg
     8761 cataataaat actgataaca tcttatagtt tgtattatat tttgtattat cgttgacatg
     8821 tataattttg atatcaaaaa ctgattttcc ctttattatt ttcgagattt attttcttaa
     8881 ttctctttaa caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag
     8941 tttaattata ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt
     9001 ttttctcatt tataaggtta aataattctc atatatcaag caaagtgaca ggcgccctta
     9061 aatattctga caaatgctct ttccctaaac tccccccata aaaaaacccg ccgaagcggg
     9121 tttttacgtt atttgcggat taacgattac tcgttatcag aaccgcccag ggggcccgag
     9181 cttaagactg gccgtcgttt tacaacacag aaagagtttg tagaaacgca aaaaggccat
     9241 ccgtcagggg ccttctgctt agtttgatgc ctggcagttc cctactctcg ccttccgctt
     9301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9721 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9781 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9841 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg gctaactacg
     9901 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9961 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10021 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10081 ctacggggtc tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag ggattttggt
    10141 catgagcttg cgccgtcccg tcaagtcagc gtaatgctct gctttt
//
