LOCUS       sequence-151055-        9280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAL4->QF2 HACK construct compatible with GMR attP integrated 
            insertions. Contains 5' and 3' GAL4 homology arms and gRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pBPGUw-HACK-G4>QF2
FEATURES             Location/Qualifiers
     source          1..9280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(141..729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(221..240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(900..1691,1692..1760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1523..1542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1761..1865)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1933..1951
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    2021..2054
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             2094..2531
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3288..3341
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(4674..4707)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4771..4892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4782..4801)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4836..4855
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5012..5722)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     5559..5577
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(5900..5933)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6968..7312
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    7322..7355
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        7371..8167
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        8187..8262
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        8263..8683
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        8704..8779
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    join(9226..9280,1..15)
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 caccgcggct tcgagaccgt gacctacatc gtcgacggta acatgtgaag tttctatact
       61 ttctagagaa tagaaacttc catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      541 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      661 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      721 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      781 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      841 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1201 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1501 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1861 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     1921 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagtacg aagtttctat
     1981 actttctaga gaatagaaac ttctacgtaa cgcgagatct gaagttccta ttctctagaa
     2041 agtataggaa cttctaggga taacagggta atagatctgg atcctaatga tgaaagctac
     2101 tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag
     2161 aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa
     2221 ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac
     2281 tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg
     2341 attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata
     2401 aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta acattgagac
     2461 agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt
     2521 tgactgtatc gattgactcg gcagctcatc atgataactc cacaattccg ttggatttta
     2581 tgcccaggga tgctcttcat ggatttgatt ggtctgaaga ggatgacatg tcggatggct
     2641 tgcccttcct gaaaacggac cccaacaata atgggttctt tggcgacggt tctctcttat
     2701 gtattcttcg atctattggc tttaaaccgg aaaattacac gaactctaac gttaacaggc
     2761 tcccgaccat gattacggat agatacacgt tggcttctag atccacaaca tcccgtttac
     2821 ttcaaagtta tctcaataat tttcacccct actgccctat cgtgcactca ccgacgctaa
     2881 tgatgttgta taataaccag attgaaatcg cgtcgaagga tcaatggcaa atccttttta
     2941 actgcatatt agccattgga gcctggtgta tagaggggga atctactgat atagatgttt
     3001 tttactatca aaatgctaaa tctcatttga cgagcaaggt cttcgagtca ggttccataa
     3061 ttttggtgac agccctacat cttctgtcgc gatatacaca gtggaggcag aaaacaaata
     3121 ctagctataa ttttcacagc ttttccataa gaatggccat atcattgggc ttgaataggg
     3181 acctcccctc gtccttcagt gatagcagca ttctggaaca aagacgccga atttggtggt
     3241 ctgtctactc ttgggagatc caattgtccc tgcttggatc ctcgcgtgag ggccgcggca
     3301 gcctgctgac ctgcggcgat gtggaggaga accccgggcc ccctaggatg ccacccaagc
     3361 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     3421 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     3481 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     3541 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     3601 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     3661 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     3721 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     3781 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     3841 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctccgtc
     3901 agttggagct ccctcctacc gccacggcta cggcctcgat aatgccgcac gtgatggagc
     3961 agcctctcag taccagcatt aaccccgtca acgaccgctt caacggtatt cccaacccca
     4021 ctccgtataa ctccgatgca gctctcgatg ctatcactca gaccaacgat tatggaagcg
     4081 taaatacaca tggtatcctc tctacttacc cgccaccggc tacgcacctt aatgaagctt
     4141 ccgtcgctct cgctcccggt ggcgcccccc cccgaccgcc tcctccgtat gttgacagca
     4201 cgaccaatca cccgccgtac cactcgaatc tggttccaat ggcgaacttt ggttactcga
     4261 ccgttgatta cgatgccatg gttgacgatt tggctagcat tgaatacacg gacgctgtgg
     4321 atgtcgaccc acagtttatg accaatctgg gattcgttcc tggatgtaac ttctccgaca
     4381 ttaatacata cgaacagtga tgacgtctat cgataccgtc gactaaagcc aaatagaaaa
     4441 ttattcagtt cctggcttaa gtttttaaaa gtgatattat ttatttggtt gtaaccaacc
     4501 aaaagaatgt aaataactaa tacataatta tgttagtttt aagttagcaa caaattgatt
     4561 ttagctatat tagctacttg gttaataaat agaatatatt tatttaaaga taattgcgtt
     4621 tttattgtca gggagtgagt ttgcttaaaa actcgtttag atccaccgca tgcataactt
     4681 cgtatagcat acattatacg aagttatggt accactcgac gattaaccgt tagtttagca
     4741 agtataattg aattttttat aagatccagc taagatacat tgatgagttt ggacaaacca
     4801 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4861 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4921 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4981 gtatggctga ttatgatcag agtcgcggcg cttacttgta cagctcgtcc atgccgccgg
     5041 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
     5101 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
     5161 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
     5221 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
     5281 agcccatggt tttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
     5341 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
     5401 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
     5461 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
     5521 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
     5581 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
     5641 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
     5701 cctcctcgcc cttgctcacc atgggccgat tgtttagctt gttcagctgc gcttgtttat
     5761 ttgcttagct ttcgcttagc gacgtgttca ctttgcttgt ttgaattgaa ttgtcgctcc
     5821 gtagacgaag cgcctctatt tatactccgg cgctaattga attagctcta attgaattag
     5881 tctctaattg aatgacgtca taacttcgta tagcatacat tatacgaagt tatagatcac
     5941 tagtcatggc atcattgaaa cagcaaggct cttacaagtt ttcacaaaaa tctatgaact
     6001 agacaaaaca gtaactgcag aaaaaagtcc tatatgtgca aaaaaatgct tgatgatttg
     6061 taatgagatt gaggaggttt cgagacaggc accaaagttt ttacaaatgg atatttccac
     6121 caccgctcta accaatttgt tgaaggaaca cccttggcta tcctttacaa gattcgaact
     6181 gaagtggaaa cagttgtctc ttatcattta tgtattaaga gattttttca ctaattttac
     6241 ccagaaaaag tcacaactag aacaggatca aaatgatcat caaagttatg aagttaaacg
     6301 atgctccatc atgttaagcg atgcagcaca aagaactgtt atgtctgtaa gtagctatat
     6361 ggacaatcat aatgtcaccc catattttgc ctggaattgt tcttattact tgttcaatgc
     6421 agtcctagta cccataaaga ctctactctc aaactcaaaa tcgaatgctg agaataacga
     6481 gaccgcacaa ttattacaac aaattaacac tgttctgatg ctattaaaaa aactggccac
     6541 ttttaaaatc cagacttgtg aaaaatacat tcaagtactg gaagaggtat gtgcgccgtt
     6601 tctgttatca cagtgtgcaa tcccattacc gcatatcagt tataacaata gtaatggtag
     6661 cgccattaaa aatattgtcg gttctgcaac tatcgcccaa taccctactc ttccggagga
     6721 aaatgtcaac aatatcagtg ttaaatatgt ttctcctggc tcagtagggc cttcacctgt
     6781 gccattgaaa tcaggagcaa gtttcagtga tctagtcaag ctgttatcta accgtccacc
     6841 ctctcgtaac tctccagtga caataccaag aagcacacct tcgcatcgct cagtcacgcc
     6901 ttttctaggg caacagcaac agctgcaatc attagtgcca ctgaccccgt ctgctttgtt
     6961 tggtggcgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
     7021 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
     7081 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
     7141 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
     7201 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
     7261 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagagt aatagactag
     7321 tgaagttcct attctctaga aagtatagga acttctagtg atctgaattc attttcaacg
     7381 tcctcgatag tatagtggtt agtatccccg cctgtcacgc gggagaccgg ggttcaattc
     7441 cccgtcgggg agaatctgtg attctttttt tttttctttt actttgttat ataaacaatt
     7501 tttgttttaa ttgaatctaa tttgccattg cttttaggaa tctcaggcat ccagcaagcg
     7561 tttgtccgcc gaatcgccca tcagtgaaga agatcctgtg gcggctacga aaatctcccc
     7621 ggccatgtcg gcctccacct ccagcgaaaa acccatcagc gagctggcca cctctgtgct
     7681 gacccaccgc tttccagact ccacctcctc acccggcgaa catggccttg gacgaatgca
     7741 gttgtcgatc cgctacagcg cccagcgtca aaaactagac gtgaccatac acaaaatcca
     7801 gaagatacca cttcgcgatc ccagcaatat ccccgatccg tatgttaagc tgtatctgtt
     7861 gcctggacgc accaaggagt cgaaacgcaa gacgagcgtg atcaaggaca actgcaaccc
     7921 cgtctacgat gcatcctttg agtacctgat ttccattgcc gaactcaggc agacggaact
     7981 ggaggtgacg gtgtgcaccc aaaagggatt cctatccggc ggtagtccca tcattggcat
     8041 ggtaggtacc cgaaagcaac cccttagtta cagacacagc gcgtacgtcc ttcgcatcct
     8101 tatgattccc aagtacatat tctgcaagag tacagtatat ataggaaaga tatccgggtg
     8161 aacttcgatg tgcagcgtac cacaacgttt tagagctaga aatagcaagt taaaataagg
     8221 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgc tcacctgtga
     8281 ttgctcctac tcaaatacaa aaacatcaaa ttttctgtca ataaagcata tttatttata
     8341 tttattttac aggaaagaat tccttttaaa gtgtatttta acctataatg aaaaacgatt
     8401 aaaaaaaata cataaaataa ttcgaaaatt tttgaatagc ccaggttgat aaaaattcat
     8461 ttcatacgtt ttataactta tgcccctaag tattttttga ccatagtgtt tcaattctac
     8521 attaatttta cagagtagaa tgaaacgcca cctactcagc caagaggcga aaaggttagc
     8581 tcgccaagca gagagggcgc cagtgctcac tactttttat aattctcaac ttctttttcc
     8641 agactcagtt cgtatatata gacctatttt caatttaacg tcgtgtattc tgagaaagct
     8701 ggagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     8761 agtggcaccg agtcggtgct tttttgccta cctggagcct gagagttgtt caataaaata
     8821 aaaatgtttc gtttttttgc tttcgccagt atttattatt tttcatcaat atgtattcaa
     8881 tttggtatgt atttagtaat tgtaatatat agacaatggt tttccgttga cgtacataca
     8941 tctgacgtgt gtttatttag acataatagt tatgttttca catcttttta atgttcgctt
     9001 aatgcgtatg cttaattaat atgttcggct tgtcgacatg cccgccgtga ccgtcgagaa
     9061 cccgctgacg ctgccccgcg tatccgcacc cgccgacgcc gtcgcacgtc ccgtgctcac
     9121 cgtgaccacc gcgcccagcg gtttcgaggg cgagggcttc ccggtgcgcc gcgcgttcgc
     9181 cgggatcaac taccgccacc tcgacccgtt catcatgatg gaccagatgg gtgaggtgga
     9241 gtacgcgccc ggggagccca agggcacgcc ctggcacccg
//
