LOCUS       sequence-151067-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB035a
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    173..191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        192..246
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    197..215
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             310..582
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             592..621
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaaacg ccatgggcat
       61 taaaaaatgc cctcttgggt tatcaagagg gtcattatat ttcgcaaaaa accccgcttc
      121 ggcggggttt tttcgcagtg ccgttaatta agtccatcga atggccagat gatccctatc
      181 agtgatagag attgacactc tatcattgat agagttattt taccactccc tatcagtgat
      241 agagaaaagt gaaatgaata gttcgacaaa aatctagacc ccctaccccc ttataaggag
      301 gaaaaaaaaa tggcacgcgt aactgttcag gacgctgtag agaaaattgg taaccgtttt
      361 gacctggtac tggtcgccgc gcgtcgcgct cgtcagatgc aggtaggcgg aaaggatccg
      421 ctggtaccgg aagaaaacga taaaaccact gtaatcgcgc tgcgcgaaat cgaagaaggt
      481 ctgatcaaca accagatcct cgacgttcgc gaacgccagg aacagcaaga gcaggaagcc
      541 gctgaattac aagccgttac cgctattgct gaaggtcgtc gtgcggccgc ggaacaaaag
      601 cttatttctg aagaggactt gagtctggaa aaacacagct ggtatcatgg ccctgtgagc
      661 cgtaacgcgg ccgaatacct gctgagctct ggcattaatg gttcttttct ggttcgtgaa
//
