LOCUS       sequence-151069-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB035h
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        207..261
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    212..230
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    242..260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             325..597
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             607..636
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
       61 aaacgccatg ggcattaaaa aatgccctct tgggttatca agagggtcat tatatttcgc
      121 aaaaaacccc gcttcggcgg ggttttttcg cagtgccgtt aattaagtcc atcgaatggc
      181 cagatgatcc ctatcagtga tagagattga cactctatca ttgatagagt tattttacca
      241 ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaaaatct agacccccta
      301 cccccttata aggaggaaaa aaaaatggca cgcgtaactg ttcaggacgc tgtagagaaa
      361 attggtaacc gttttgacct ggtactggtc gccgcgcgtc gcgctcgtca gatgcaggta
      421 ggcggaaagg atccgctggt accggaagaa aacgataaaa ccactgtaat cgcgctgcgc
      481 gaaatcgaag aaggtctgat caacaaccag atcctcgacg ttcgcgaacg ccaggaacag
      541 caagagcagg aagccgctga attacaagcc gttaccgcta ttgctgaagg tcgtcgtgcg
      601 gccgcggaac aaaagcttat ttctgaagag gacttgggca gctctgtgag tagcgttccg
      661 accaaactgg aagtggttgc agcaaccccg acgagcctgc tgatttcttg ggatgccccg
      721 atgtctagta gctctgtgta ttactatcgt atcacctacg gtgaa
//
