LOCUS       sequence-151072-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB042a
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        206..260
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    211..229
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    241..259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             324..596
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             606..635
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
       61 aacgccatgg gcattaaaaa atgccctctt gggttatcaa gagggtcatt atatttcgca
      121 aaaaaccccg cttcggcggg gttttttcgc agtgccgtta attaagtcca tcgaatggcc
      181 agatgatccc tatcagtgat agagattgac actctatcat tgatagagtt attttaccac
      241 tccctatcag tgatagagaa aagtgaaatg aatagttcga caaaaatcta gaccccctac
      301 ccccttataa ggaggaaaaa aaaatggcac gcgtaactgt tcaggacgct gtagagaaaa
      361 ttggtaaccg ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag
      421 gcggaaagga tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg
      481 aaatcgaaga aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc
      541 aagagcagga agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgtgcgg
      601 ccgcggaaca aaagcttatt tctgaagagg acttgggcag ctctggtagt agcgtgccga
      661 ccaacctgga agtggttgcg gccaccccga cgagtctgct gattagctgg gatgcgtact
      721 ctagtagcta ctacgtttac tactaccgta tcacctatg
//
