LOCUS       sequence-151077-        8953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Liberti WA 3rd, Markowitz JE, Perkins LN, Liberti DC, Leman DP, 
            Guitchounts G, Velho T, Kotton DN, Lois C, Gardner TJ
  TITLE     Unstable neurons underlie a stable learned behavior.
  JOURNAL   Nat Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405.
  PUBMED    27723744
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405."
COMMENT     Sequence Label: pHAGE-RSV-tdTomato-2A-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..8953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2479
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     regulatory      2483..2492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2489..3916
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             3929..3982
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3983..3991,3992..4009,4010..4012,4013..4045,4046..4048,
                     4049..4072,4073..4099,4100..4156,4157..4162,4163..4432,
                     4433..4450,4451..4885,4886..4891,4892..5335)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4079..4088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4369..4390
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4496..4517)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4757..4776)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5343..5931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5396..5416)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5814..5825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6006..6239
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6358..6376)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6444..6548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6549..6617,6618..7409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6767..6786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7580..8168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8069..8088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8322..8339
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8579..8600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8615..8620,8621..8638,8639..8645)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8653..8669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8658..8680
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8677..8693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8677..8693
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8743..8762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca ataaacgcca tttgaccatt
     2461 caccacattg gtgtgcaccg cggccgccat ggtgagcaag ggcgaggagg tcatcaaaga
     2521 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     2581 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     2641 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     2701 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     2761 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     2821 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     2881 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     2941 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     3001 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     3061 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     3121 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
     3181 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
     3241 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     3301 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3361 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     3421 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     3481 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     3541 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     3601 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     3661 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     3721 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     3781 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     3841 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     3901 ggacgagctg tacaagtctg gcagcggaga gggcagagga agtcttctaa catgcggtga
     3961 cgtggaggag aatcccggcc ctatgggttc tcatcatcat catcatcatg gtatggctag
     4021 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc
     4081 caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat
     4141 aggtcggctg agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat
     4201 caaggcgaac ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
     4261 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4321 gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4381 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
     4441 cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4501 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
     4561 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4621 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     4681 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
     4741 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     4801 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4861 cctggggcac aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga
     4921 atttaaagag gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga
     4981 gctggggacg gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat
     5041 gatcaatgaa gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat
     5101 gatggcaaga aaaatgaaat acagggacac ggaagaagaa attagagaag cgttcggtgt
     5161 gtttgataag gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa
     5221 ccttggagag aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga
     5281 tggggatggt caggtaaact acgaagagtt tgtacaaatg atgacagcga agtgaggatc
     5341 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5401 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5461 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5521 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5581 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5641 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5701 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5761 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5821 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5881 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     5941 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6001 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6061 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6121 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6181 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6241 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6301 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     6361 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6781 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7021 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8281 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8341 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8401 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8461 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8521 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8581 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8641 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     8701 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8761 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     8821 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     8881 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     8941 caaacttagt agt
//
