LOCUS       sequence-151081-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB042d
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        208..262
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    213..231
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    243..261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             326..598
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             608..637
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
       61 taaacgccat gggcattaaa aaatgccctc ttgggttatc aagagggtca ttatatttcg
      121 caaaaaaccc cgcttcggcg gggttttttc gcagtgccgt taattaagtc catcgaatgg
      181 ccagatgatc cctatcagtg atagagattg acactctatc attgatagag ttattttacc
      241 actccctatc agtgatagag aaaagtgaaa tgaatagttc gacaaaaatc tagaccccct
      301 acccccttat aaggaggaaa aaaaaatggc acgcgtaact gttcaggacg ctgtagagaa
      361 aattggtaac cgttttgacc tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt
      421 aggcggaaag gatccgctgg taccggaaga aaacgataaa accactgtaa tcgcgctgcg
      481 cgaaatcgaa gaaggtctga tcaacaacca gatcctcgac gttcgcgaac gccaggaaca
      541 gcaagagcag gaagccgctg aattacaagc cgttaccgct attgctgaag gtcgtcgtgc
      601 ggccgcggaa caaaagctta tttctgaaga ggacttgggc agcgatctgg gtaaaaaact
      661 gatggaagcg gcccgtgcag gccaggatga tgaagtgcgc attctgatgg cgaacggcgc
      721 cgatgttaat gccaccgatt attggggtaa cacgccgctg catctggcag cgtggtctgg
      781 tcgtctggaa attgtggaa
//
