LOCUS       sequence-151097-         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB076i6
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(94..111)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      264..350
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      442..469
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaaac gccatgggca
       61 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
      121 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
      181 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
      241 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      301 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
      361 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
      421 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
      481 aaactcgctg aggattctag agcacagcta acaccacgtc gtccctatct gctgccctag
      541 gtctatgagt ggttgctgga taactttacg ggcatgcata aggctcggta tctatattca
      601 gggagaccac aacggtttcc ctctacaaat aatt
//
