LOCUS       sequence-151124-        9236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding inducible shBrd9_1061 
            (targeting murine Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRE...
FEATURES             Location/Qualifiers
     source          1..9236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(71..90)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(112..441)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      126..261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     426..446
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     582..604
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             707..1132
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     CDS             complement(1271..2065)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1271..2065)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1382..1401)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1992..2011
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    2081..2654
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2241..2260)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2470..2487
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(2690..3403,3404..3406,3407..3409))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2735..2756)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3084..3103
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3343..3364
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3432..3931)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3480..3499)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3873..3896
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     ncRNA           complement(3954..4080)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(4108..4122)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(4150..4271)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(4340..5017)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(4402..4425)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     4817..4837
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     complement(5095..5119)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5116..5154)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(5187..5205,5206..5228,5229..5247,5248..5270,5271..5289,
                     5290..5312,5313..5331,5332..5354,5355..5373,5374..5396,
                     5397..5415,5416..5438,5439..5457)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             complement(5507..5923)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5523..5539)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5555..5577)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6124..6323
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6386..6561
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(6562..6765)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6766..7145
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7245..7263)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7331..7435
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7436..7504,7505..8296)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7654..7673)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8467..9055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8956..8975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9209..9226
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtaagct ttttgcaaaa
      121 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
      181 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
      241 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
      301 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
      361 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
      421 tccacaccct aactgacaca cattccacag ccggatctac actccgctat cgctacgtga
      481 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      541 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      601 gaggttttca ccgtcatcac cgaaacgcgc gaggcagcca gcttacctcc cggtggtggg
      661 tcggtggtcc ctgggcaggg gtctcccgat cccggacgag cccccaaatg aaagaccccc
      721 gctgacgggt agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc
      781 ggatgcaact gcaagagggt ttattggata cacgggtacc cgggcgactc agtcaatcgg
      841 aggactggcg ccccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc
      901 gcgcgaacag aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt
      961 caggtccttg gggcaccctg gaaacatctg atggttctct agggtcgacc ctgttccatc
     1021 tgttcctgac cttgatctga acttctctat tctcagttat gtatttttcc atgccttgca
     1081 aaatggcgtt acttaagcta gcttgccaaa cctacaggtg gggtctttca ttcccccctt
     1141 tttctggaga ctaaataaaa tcttttattt tatctatggc tcgtactcta taggcttcag
     1201 ctggtgatat tgttgagtca aaactagagc ctggaccact gatatctcga cggtatcgat
     1261 gcatcgtaca tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     1321 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     1381 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     1441 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     1501 catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt
     1561 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     1621 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     1681 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     1741 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     1801 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     1861 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     1921 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     1981 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     2041 cgtgcaatcc atcttgttca atcatggtgg accggtaagc ttatcatcgt gtttttcaaa
     2101 ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac
     2161 atgtaaagca tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg
     2221 catccttcag ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta
     2281 tccaactcac aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg
     2341 gcttttggcc gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg
     2401 ctacagacgt tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca
     2461 acagaccttg cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga
     2521 cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca
     2581 tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg
     2641 gggagggaga gggggggggg ggggggagag gggcggaatt gaacaattct tacttgtaca
     2701 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     2761 cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag tggttgtcgg
     2821 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     2881 cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct
     2941 tgtcggcggt gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     3001 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     3061 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     3121 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     3181 cggggtagcg ggcgaagcac tgcaggccgt agcccagggt ggtcacgagg gtgggccagg
     3241 gcacgggcag cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat
     3301 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     3361 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggaagctt
     3421 gggctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc
     3481 ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg
     3541 agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg
     3601 aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg
     3661 gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac
     3721 gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg
     3781 aaggggccac caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga
     3841 ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca
     3901 gactgccttg ggaaaagcgc ctcccctacc cggtagaatt gctagaattg aaaaaagtga
     3961 tttaatttat accattttaa ttcagctttg taaaaatgta tcaaagagat agcaaggtat
     4021 tcagttttag taaacaagat aattgctcct aaagtagccc cttgaattcc gaggcagtag
     4081 gcatggacct gagttcactg tctaatacat ctgtggcttc actattagac agtgaactca
     4141 ggtccgcgct cactgtcaac agcaatatac cttctcgagc cttctgttgg gttaacctga
     4201 agaagtaatc ccagcaagtg tttccaagat gtgcaggcaa cgattctgta aagtactgaa
     4261 gcctcattca aacaattacc ctgttatccc tagtcgagag atcttatcat atgactagta
     4321 aattctagag tcgcggccgc tacaggaaca ggtggtggcg gccctcggtg cgctcgtact
     4381 gctccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggcg tccacgtagt
     4441 agtagccggg cagctgcacg ggcttcttgg ccatgtagat ggacttgaac tccaccaggt
     4501 agtggccgcc gtccttcagc ttcagggcct tgtgggtctc gcccttcagc acgccgtcgc
     4561 gggggtacag gcgctcggtg gaggcctccc agcccatggt cttcttctgc atcacggggc
     4621 cgtcggaggg gaagttcacg ccgatgaact tcaccttgta gatgaagcag ccgtcctgca
     4681 gggaggagtc ctgggtcacg gtcgccacgc cgccgtcctc gaagttcatc acgcgctccc
     4741 acttgaagcc ctcggggaag gacagcttct tgtagtcggg gatgtcggcg gggtgcttca
     4801 cgtacacctt ggagccgtac tggaactggg gggacaggat gtcccaggcg aagggcaggg
     4861 ggccgccctt ggtcaccttc agcttcacgg tgttgtggcc ctcgtagggg cggccctcgc
     4921 cctcgccctc gatctcgaac tcgtggccgt tcacggtgcc ctccatgcgc accttgaagc
     4981 gcatgaactc ggtgatgacg ttctcggagg aggccatggt ggatccgtcg agaggctgga
     5041 tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg
     5101 acggttcact aaacgagctc tgcttatata ggcctcccac cgtacacgcc tacctcgacc
     5161 cgggtaccga gctcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
     5221 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
     5281 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
     5341 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
     5401 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
     5461 gtaaactcga tgctctagag gatcgatccc ccagatcttc aattccggcg cctagagaag
     5521 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
     5581 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
     5641 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
     5701 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
     5761 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
     5821 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
     5881 agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt tcagacaaat
     5941 acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc agcagacaag
     6001 acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
     6061 ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac atcgactccc
     6121 ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc ccagggttgc
     6181 ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag agctagttag
     6241 ctaactagta cagacgcagg cgcataacat caaacataga cactagacaa tcggacagac
     6301 acagataagt tgctggccag cttacctccc ggtggtgggt cggtggtccc tgggcagggg
     6361 tctccaaatc ccggacgagc ccccaaatga aagacccccg tcgtgggtag tcaatcactc
     6421 agaggagacc ctcccaagga acagcgagac cacgattcgg atgcaaacag caagaggctt
     6481 tattgggaat acgggtaccc gggcgacgca gtctatcgga agactggcgc gccgagtgag
     6541 gggttgtggg ctcttttatt gagctctgct tatatagacc tcccaccgta cacgcctacc
     6601 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     6661 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     6721 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     6781 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     6841 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     6901 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     6961 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     7021 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     7081 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     7141 atgtcaacat ggcggtaatg ttggacatga gccaatataa atgtacatat tatgatatgg
     7201 atacaacgta tgcaatggcc aagctaattc ttgaagacga aagggcctcg tgatacgcct
     7261 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7321 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7381 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7441 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7501 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7561 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7621 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     7681 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7741 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7801 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7861 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7921 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     7981 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8041 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8101 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8161 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8221 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8281 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8341 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8401 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8461 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8521 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8581 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8641 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8701 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8761 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8821 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8881 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8941 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9001 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9061 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
