LOCUS       sequence-151131-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing PrM from 
            Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_PrM_Flag
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(193..216)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      732..741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcaagaaga cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg
       61 gaaaataaca tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg
      121 ttaggggggg gggagggaga ggggcggaat ttgcggccgc taagcttcac gcgtcgatat
      181 cctcgagtgc tacttgtcat cgtcgtcttt gtagtcggcc atcccggagc tgtatgccgg
      241 ggcaatcagc agtatcatga ccaagtatat gactttttgg ctcgttgagc ttcccaaaag
      301 ccaagcgatg gcagctgctg ctaacgcgaa gccagggttc ctgaatatcc aattttcgac
      361 tctaatcaag tgctttgtgt attctcttga ttccaaccag gtttgcgacc gcgtttgcag
      421 cttcctggtg gaatgggagg ggagcgtcac agctcttcta gatctccgtg cttcaccttt
      481 tttgtgatgg caggttccgt acacaaccca agttgacgtc gtgttgcacc aacaatcgac
      541 gtcatctggt tccaccccct catccagcat agggcattca tagctcatgg tggcatcaca
      601 catgtgtcca agatccatga tctgtatata acacttattc atccccaatg tggttggaaa
      661 agatatggcc tccccagcat cgtttctgtc caagtacata tagtatgcac tcccacgtct
      721 agtgacctcc gccatggtgg cgaccggtgg taggcgccgg tcacagcttg gatctgtaac
      781 ggcgcagaac agaaaacgaa acaaagacgt agagttgagc aagcagggtc aggcaaagcg
      841 tggagagccg gctgagtcta ggtaggctcc aagggagcgc cggacaaagg cccggtctcg
      901 acctgagctt taaacttacc tagacggcgg acgcagttca ggaggcacca caggcgggag
      961 gcggcagaac gcgactcaac cggcgtgg
//
