LOCUS       sequence-151137-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV PIG (Puro IRES GFP)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(203..802)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     783..802
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
       61 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
      121 aacgttaggg ggggggggcg gaattcgatg catggggtcg tgcgctcctt tcggtcgggc
      181 gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg
      241 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg taaaagggga
      301 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
      361 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga
      421 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
      481 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
      541 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt
      601 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
      661 cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg
      721 cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca
      781 ccgtgggctt gtactcggtc atggtaagct tgggctgcag gtcgaaaggc ccggagatga
      841 ggaagaggag aa
//
