LOCUS       sequence-151138-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human NEK8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: NEK8 gRNA (BRDN0001148289)
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..136
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        216..291
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    347..464
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
       61 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      121 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      181 aaggacgaaa caccgcaggc atacactgac gacaggtttt agagctagaa atagcaagtt
      241 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttaag
      301 cttggcgtaa ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      481 acagcagaga tccactttgg cgccggctcg agggggcccg ggtgcaaaga tggataaagt
      541 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      601 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      661 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
      721 gtgatgtcgt gtactggctc cgcctttt
//
