LOCUS       sequence-151145-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) 
            protein and blasticidin resistance from EFS promoter. Lentiviral 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiCas9n(D10A)-Blast
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
       61 tcgcaacggg tttgccgcca gaacacagga ccggttctag agcgctgcca ccatggacaa
      121 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
      181 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      241 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      301 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      361 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      421 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
      481 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
      541 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
      601 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
      661 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
      721 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
      781 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc
      841 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
      901 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
      961 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     1021 cgacatcctg agagtgaaca ccgagatcac caaggcccc
//
