LOCUS       sequence-151148-         456 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 behind constitutive promoter J23103 in pSEVA331Bb backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: J23103-mRFP1-331Bb
FEATURES             Location/Qualifiers
     source          1..456
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             150..161
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(170..204)
                     /label=BBa_J23112
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0"
     promoter        complement(170..204)
                     /label=BBa_J23103
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
     misc_feature    205..226
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(228..250)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      265..351
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt
       61 tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg
      121 ataacgtctt cggaggaagc catctagtat ttctcctctt tcactagtag ctagcataat
      181 ccctaggact gagctagcta tcagctctag aagcggccgc gaattctcct gtgtgaaatt
      241 gttatccgct ttaattaaag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      301 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      361 tagacagctg ggcgcgcccc ccctacgggc ttgctctccg ggcttcgccc tgcgcggtcg
      421 ctgcgctccc ttgccagccc gtggatatgt ggacga
//
