LOCUS       sequence-151150-         467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: J23104-mRFP1-331Bb
FEATURES             Location/Qualifiers
     source          1..467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             161..172
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(181..215)
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     misc_feature    216..237
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(239..261)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      276..362
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cacctttggt aactttcagt ttagcggtct gggtaccttc gtacggacga ccttcacctt
       61 caccttcgat ttcgaactcg tgaccgttaa cggaaccttc catacgaact ttgaaacgca
      121 tgaactcttt gataacgtct tcggaggaag ccatctagta tttctcctct ttcactagta
      181 gctagcacaa tacctaggac tgagctagct gtcaactcta gaagcggccg cgaattctcc
      241 tgtgtgaaat tgttatccgc tttaattaaa ggcatcaaat aaaacgaaag gctcagtcga
      301 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      361 atccgccgcc ctagacagct gggcgcgccc cccctacggg cttgctctcc gggcttcgcc
      421 ctgcgcggtc gctgcgctcc cttgccagcc cgtggatatg tggacga
//
