LOCUS       sequence-151160-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector encoding shRNA and mCherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Rojansky R, Cha MY, Chan DC
  TITLE     Elimination of paternal mitochondria in mouse embryos occurs through
            autophagic degradation dependent on PARKIN and MUL1.
  JOURNAL   Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
  PUBMED    27852436
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-17"; volume: "5. pii: e17896. doi"; pages: " 
            10.7554/eLife.17896"
COMMENT     Sequence Label: pFUChW nontargeting shRNA
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    108..225
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(338..551)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(398..417)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
       61 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
      121 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      181 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      241 gacagcagag atccagtttg gttaattaag aattcaaaaa gactagaagg cacagaggga
      301 tctcttgaat ccctctgtgc cttctagtcg gatccctgtg gtctcataca gaacttataa
      361 gattcccaaa tccaaagaca tttcacgttt atggtgattt cccagaacac atagcgacat
      421 gcaaatattg cagggcgcca ctcccctgtc cctcacagcc atcttcctgc cagggcgcac
      481 gcgcgctggg tgttcccgcc tagtgacact gggcccgcga ttccttggag cgggttgatg
      541 acgtcagcgt ttaattaacc cgtgtcggct ccagatctgg cctccgcgcc gggttttggc
      601 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
      661 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
      721 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
      781 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
      841 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
      901 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
      961 ctgtgatcgt c
//
