LOCUS       sequence-151161-        9948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression MbCpf1 nuclease and Mb crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4495
FEATURES             Location/Qualifiers
     source          1..9948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(220..468)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(278..297)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(448..468)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        521..900
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        901..1104
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1055..1075
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1149..1168
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1149..1167
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5334..5381
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5388..5468
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5542..5559)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5548..5772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5818..6246
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5905..5924)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6115..6136
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6255..6275)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6260..6589
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6440..6575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6502..6521
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6656..7450
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6710..6729)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7320..7339
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7624..7745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7661..7680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7715..7734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7794..7810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7794..7810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7807..7829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7818..7834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7842..7848,7849..7866,7867..7872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7887..7908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8025..8042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8196..8781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8276..8295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8952..9743,9744..9812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9575..9594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9813..9917)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcgta caaaaaagga
      181 gacgttcgaa cgtctccatc tacaaacagt agaaatttcg gtgtttcgtc ctttccacaa
      241 gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat
      301 tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt
      361 attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct ctctaacagc
      421 cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctt cctgcccgac
      481 cttagatccg aatccatatc gatatgctag ttggcgcgtt gacattgatt attgactagt
      541 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      601 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      661 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      721 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      781 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      841 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      901 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      961 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     1021 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     1081 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     1141 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
     1201 ttggtaccgc caccatgctg ttccaggact ttacccacct gtatccactg tccaagacag
     1261 tgagatttga gctgaagccc atcgatagga ccctggagca catccacgcc aagaacttcc
     1321 tgtctcagga cgagacaatg gccgatatgc accagaaggt gaaagtgatc ctggacgatt
     1381 accaccgcga cttcatcgcc gatatgatgg gcgaggtgaa gctgaccaag ctggccgagt
     1441 tctatgacgt gtacctgaag tttcggaaga acccaaagga cgatgagctg cagaagcagc
     1501 tgaaggatct gcaggccgtg ctgagaaagg agatcgtgaa gcccatcggc aatggcggca
     1561 agtataaggc cggctacgac aggctgttcg gcgccaagct gtttaaggac ggcaaggagc
     1621 tgggcgatct ggccaagttc gtgatcgcac aggagggaga gagctcccca aagctggccc
     1681 acctggccca cttcgagaag ttttccacct atttcacagg ctttcacgat aaccggaaga
     1741 atatgtattc tgacgaggat aagcacaccg ccatcgccta ccgcctgatc cacgagaacc
     1801 tgccccggtt tatcgacaat ctgcagatcc tgaccacaat caagcagaag cactctgccc
     1861 tgtacgatca gatcatcaac gagctgaccg ccagcggcct ggacgtgtct ctggccagcc
     1921 acctggatgg ctatcacaag ctgctgacac aggagggcat caccgcctac aatacactgc
     1981 tgggaggaat ctccggagag gcaggctctc ctaagatcca gggcatcaac gagctgatca
     2041 attctcacca caaccagcac tgccacaaga gcgagagaat cgccaagctg aggccactgc
     2101 acaagcagat cctgtccgac ggcatgagcg tgtccttcct gccctctaag tttgccgacg
     2161 atagcgagat gtgccaggcc gtgaacgagt tctatcgcca ctacgccgac gtgttcgcca
     2221 aggtgcagag cctgttcgac ggctttgacg atcaccagaa ggatggcatc tacgtggagc
     2281 acaagaacct gaatgagctg tccaagcagg ccttcggcga ctttgcactg ctgggacgcg
     2341 tgctggacgg atactatgtg gatgtggtga atccagagtt caacgagcgg tttgccaagg
     2401 ccaagaccga caatgccaag gccaagctga caaaggagaa ggataagttc atcaagggcg
     2461 tgcactccct ggcctctctg gagcaggcca tcgagcacta taccgcaagg cacgacgatg
     2521 agagcgtgca ggcaggcaag ctgggacagt acttcaagca cggcctggcc ggagtggaca
     2581 accccatcca gaagatccac aacaatcaca gcaccatcaa gggctttctg gagagggagc
     2641 gccctgcagg agagagagcc ctgccaaaga tcaagtccgg caagaatcct gagatgacac
     2701 agctgaggca gctgaaggag ctgctggata acgccctgaa tgtggcccac ttcgccaagc
     2761 tgctgaccac aaagaccaca ctggacaatc aggatggcaa cttctatggc gagtttggcg
     2821 tgctgtacga cgagctggcc aagatcccca ccctgtataa caaggtgaga gattacctga
     2881 gccagaagcc tttctccacc gagaagtaca agctgaactt tggcaatcca acactgctga
     2941 atggctggga cctgaacaag gagaaggata atttcggcgt gatcctgcag aaggacggct
     3001 gctactatct ggccctgctg gacaaggccc acaagaaggt gtttgataac gcccctaata
     3061 caggcaagag catctatcag aagatgatct ataagtacct ggaggtgagg aagcagttcc
     3121 ccaaggtgtt cttttccaag gaggccatcg ccatcaacta ccacccttct aaggagctgg
     3181 tggagatcaa ggacaagggc cggcagagat ccgacgatga gcgcctgaag ctgtatcggt
     3241 ttatcctgga gtgtctgaag atccacccta agtacgataa gaagttcgag ggcgccatcg
     3301 gcgacatcca gctgtttaag aaggataaga agggcagaga ggtgccaatc agcgagaagg
     3361 acctgttcga taagatcaac ggcatctttt ctagcaagcc taagctggag atggaggact
     3421 tctttatcgg cgagttcaag aggtataacc caagccagga cctggtggat cagtataata
     3481 tctacaagaa gatcgactcc aacgataatc gcaagaagga gaatttctac aacaatcacc
     3541 ccaagtttaa gaaggatctg gtgcggtact attacgagtc tatgtgcaag cacgaggagt
     3601 gggaggagag cttcgagttt tccaagaagc tgcaggacat cggctgttac gtggatgtga
     3661 acgagctgtt taccgagatc gagacacgga gactgaatta taagatctcc ttctgcaaca
     3721 tcaatgccga ctacatcgat gagctggtgg agcagggcca gctgtatctg ttccagatct
     3781 acaacaagga cttttcccca aaggcccacg gcaagcccaa tctgcacacc ctgtacttca
     3841 aggccctgtt ttctgaggac aacctggccg atcctatcta taagctgaat ggcgaggccc
     3901 agatcttcta cagaaaggcc tccctggaca tgaacgagac aacaatccac agggccggcg
     3961 aggtgctgga gaacaagaat cccgataatc ctaagaagag acagttcgtg tacgacatca
     4021 tcaaggataa gaggtacaca caggacaagt tcatgctgca cgtgccaatc accatgaact
     4081 ttggcgtgca gggcatgaca atcaaggagt tcaataagaa ggtgaaccag tctatccagc
     4141 agtatgacga ggtgaacgtg atcggcatcg atcggggcga gagacacctg ctgtacctga
     4201 ccgtgatcaa tagcaagggc gagatcctgg agcagtgttc cctgaacgac atcaccacag
     4261 cctctgccaa tggcacacag atgaccacac cttaccacaa gatcctggat aagagggaga
     4321 tcgagcgcct gaacgcccgg gtgggatggg gcgagatcga gacaatcaag gagctgaagt
     4381 ctggctatct gagccacgtg gtgcaccaga tcagccagct gatgctgaag tacaacgcca
     4441 tcgtggtgct ggaggacctg aatttcggct ttaagagggg ccgctttaag gtggagaagc
     4501 agatctatca gaacttcgag aatgccctga tcaagaagct gaaccacctg gtgctgaagg
     4561 acaaggccga cgatgagatc ggctcttaca agaatgccct gcagctgacc aacaatttca
     4621 cagatctgaa gagcatcggc aagcagaccg gcttcctgtt ttatgtgccc gcctggaaca
     4681 cctctaagat cgaccctgag acaggctttg tggatctgct gaagccaaga tacgagaaca
     4741 tcgcccagag ccaggccttc tttggcaagt tcgacaagat ctgctataat gccgacaagg
     4801 attacttcga gtttcacatc gactacgcca agtttaccga taaggccaag aatagccgcc
     4861 agatctggac aatctgttcc cacggcgaca agcggtacgt gtacgataag acagccaacc
     4921 agaataaggg cgccgccaag ggcatcaacg tgaatgatga gctgaagtcc ctgttcgccc
     4981 gccaccacat caacgagaag cagcccaacc tggtcatgga catctgccag aacaatgata
     5041 aggagtttca caagtctctg atgtacctgc tgaaaaccct gctggccctg cggtacagca
     5101 acgcctcctc tgacgaggat ttcatcctgt cccccgtggc aaacgacgag ggcgtgttct
     5161 ttaatagcgc cctggccgac gatacacagc ctcagaatgc cgatgccaac ggcgcctacc
     5221 acatcgccct gaagggcctg tggctgctga atgagctgaa gaactccgac gatctgaaca
     5281 aggtgaagct ggccatcgac aatcagacct ggctgaattt cgcccagaac aggaaaaggc
     5341 cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac ccatacgatg
     5401 ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg
     5461 actatgccta agaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
     5521 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5581 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5641 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5701 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5761 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5821 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5881 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5941 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6001 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6061 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6121 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6181 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6241 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6301 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6361 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6421 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6481 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6541 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6601 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6661 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6721 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6781 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6841 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6901 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6961 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7021 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7081 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7141 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     7201 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7261 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7321 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7381 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7441 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7501 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7561 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7621 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7681 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7741 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7801 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7861 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7921 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7981 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8041 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8101 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8161 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8221 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8281 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8341 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8401 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8461 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8521 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8581 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8641 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8701 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg
     8761 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8821 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
